Swart K, Debets A J, Kobus G, Bos C J
Department of Genetics, Agricultural University, Wageningen, The Netherlands.
Antonie Van Leeuwenhoek. 1992 May;61(4):259-64. doi: 10.1007/BF00713933.
Aspergillus niger mutants defective in arginine or proline biosynthesis have been isolated and 12 genetic loci were identified. Mutation was induced by low doses UV, and mutants were isolated after filtration enrichment. The mutants were classified according to their phenotype in growth tests and were further characterized in complementation tests. The arginine auxotrophic mutants represent nine complementation groups. Three additional complementation groups were found for mutants that could grow on proline (two of them on arginine too). Linkage group analysis was done in somatic diploids obtained from a mutant and a master strain with genetic markers on six chromosomes. The arg genes belong to six different linkage groups and the pro genes to two. One arg-mutant could be complemented by transformation with the A. nidulans argB+ gene, and this A. niger gene thus appeared to be homologous to the A. nidulans argB. We isolated an A. niger strain with the argB gene tightly linked with the nicA1 marker. This strain is very suitable as acceptor for transformation with an argB-plasmid, because transformants with inserts on the homologous site can be recognized and analyzed genetically using the nicA1 marker gene.
已分离出精氨酸或脯氨酸生物合成存在缺陷的黑曲霉突变体，并鉴定出12个基因位点。通过低剂量紫外线诱导突变，经过滤富集后分离出突变体。根据其在生长试验中的表型对突变体进行分类，并在互补试验中进一步表征。精氨酸营养缺陷型突变体代表9个互补群。对于能够在脯氨酸上生长的突变体（其中两个也能在精氨酸上生长），又发现了另外3个互补群。在从一个突变体和一个在六条染色体上带有遗传标记的野生型菌株获得的体细胞二倍体中进行连锁群分析。精氨酸基因属于6个不同的连锁群，脯氨酸基因属于2个。一个精氨酸突变体可以通过用构巢曲霉argB⁺基因转化来互补，因此这个黑曲霉基因似乎与构巢曲霉argB同源。我们分离出了一个精氨酸基因与烟酰胺腺嘌呤二核苷酸磷酸（NADP）标记紧密连锁的黑曲霉菌株。该菌株非常适合作为用精氨酸B质粒转化的受体，因为可以使用烟酰胺腺嘌呤二核苷酸磷酸（NADP）标记基因在遗传上识别和分析在同源位点带有插入片段的转化体。
