Swart K, van de Vondervoort P J, Witteveen C F, Visser J
Department of Genetics, Agricultural University, Wageningen, The Netherlands.
Curr Genet. 1990 Dec;18(5):435-9. doi: 10.1007/BF00309913.
A number of mutants of Aspergillus niger, affected in glucose oxidase (GOX) expression, are described. The overproducing mutants could be classified into seven complementation groups whereas two glucose oxidase-negative complementation groups were recognized. These nine gox loci were assigned to linkage groups using master strains with marked chromosomes. Three gox loci are in linkage group II, one is in III, two are in V and two are in linkage group VII. One weak glucose oxidase-overproducing mutant could not be assigned to one of the linkage groups. These genetically well characterized mutants will be used in a strain improvement program based on genetic recombination.
本文描述了一些黑曲霉突变体，这些突变体在葡萄糖氧化酶（GOX）表达方面存在缺陷。高产突变体可分为七个互补群，同时识别出两个葡萄糖氧化酶阴性互补群。利用带有标记染色体的主菌株，将这九个gox基因座定位到连锁群上。三个gox基因座位于连锁群II，一个位于III，两个位于V，两个位于连锁群VII。一个弱葡萄糖氧化酶高产突变体无法定位到任何一个连锁群中。这些遗传特征明确的突变体将用于基于基因重组的菌株改良计划。
