Audet I, Laforest J P, Martineau G P, Matte J J
Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, Lennoxville, Quebec, J1M 1Z3 Canada.
J Anim Sci. 2004 Feb;82(2):626-33. doi: 10.2527/2004.822626x.
The aim of the present study was to determine the effects of dietary supplements of vitamins on vitamin status, libido, and semen characteristics in young boars under normal and intensive semen collection. Sixty Landrace, Yorkshire, and Duroc boars were allocated randomly from 6 to 10 mo of age to one of the following diets: 1) basal diet (industry level) for minerals and vitamins (Control, n = 15); 2) basal diet supplemented with vitamin C (ASC, n = 15); 3) basal diet supplemented with fat-soluble vitamins (FSV, n = 15); and 4) basal diet supplemented with water-soluble vitamins (WSV, n = 15). After puberty (approximately 12 mo of age), semen was collected at a regular frequency (three times every 2 wk) for 5 wk. Thereafter, all boars were intensively collected (daily during 2 wk). A recovery period (semen collection three times every 2 wk) followed and lasted for 10 wk. Sperm quality (percentage of motile cells and percentage of morphologically normal cells) and quantity (sperm concentration, semen volume, and total sperm number) were recorded as well as direct and hormone related measurements of boar libido. Blood and seminal plasma samples were taken to monitor vitamin status. High concentrations of B6 (P < 0.05) and folic acid (P < 0.05) were observed in the blood plasma of WSV boars, whereas greater concentrations of vitamin E (P < 0.01) were obtained in FSV boars. In the seminal plasma, folic acid concentrations tended to be greater in WSV boars (P < 0.08). During the intensive collection period, there was a tendency (P < 0.06) for semen production to be greater in WSV boars, the effect being less pronounced (P < 0.10) in FSV boars. During the recovery period, the percentage of motile sperm cells was greater in WSV boars (P < 0.03) and, to a lesser extent, in FSV boars (P < 0.10) compared with Control boars. Sperm morphology and libido were not affected by treatments. These results indicate that the transfer of vitamins from blood to seminal plasma is limited and the dietary supplements of water-soluble and fat-soluble vitamins may increase semen production during intensive semen collection.
本研究的目的是确定在正常和密集采精条件下，日粮补充维生素对青年公猪维生素状态、性欲和精液特性的影响。将60头长白、约克夏和杜洛克公猪在6至10月龄时随机分配到以下日粮之一：1）矿物质和维生素的基础日粮（工业水平，对照组，n = 15）；2）补充维生素C的基础日粮（ASC组，n = 15）；3）补充脂溶性维生素的基础日粮（FSV组，n = 15）；4）补充水溶性维生素的基础日粮（WSV组，n = 15）。青春期后（约12月龄），以固定频率（每2周3次）采精5周。此后，所有公猪进行密集采精（2周内每天采精）。随后是恢复期（每2周采精3次），持续10周。记录精子质量（活动细胞百分比和形态正常细胞百分比）和数量（精子浓度、精液体积和总精子数）以及公猪性欲的直接和激素相关测量值。采集血液和精浆样本以监测维生素状态。在WSV组公猪的血浆中观察到高浓度的B6（P < 0.05）和叶酸（P < 0.05），而FSV组公猪获得了更高浓度的维生素E（P < 0.01）。在精浆中，WSV组公猪的叶酸浓度有升高趋势（P < 0.08）。在密集采精期，WSV组公猪的精液产量有升高趋势（P < 0.06），FSV组公猪的这种影响不太明显（P < 0.10）。在恢复期，与对照组公猪相比，WSV组公猪的活动精子细胞百分比更高（P < 0.03），FSV组公猪在较小程度上也更高（P < 0.10）。精子形态和性欲不受处理的影响。这些结果表明，维生素从血液向精浆的转移是有限的，日粮补充水溶性和脂溶性维生素可能会增加密集采精期间的精液产量。
