Galić Ivan, Dragin Saša, Stančić Ivan, Maletić Milan, Apić Jelena, Kladar Nebojša, Spasojević Jovan, Grba Jovana, Kovačević Zorana
Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Serbia.
Department for Animal Sciences, Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Serbia.
Animals (Basel). 2022 May 18;12(10):1301. doi: 10.3390/ani12101301.
The study was conducted on a commercial pig farm located in Serbia. Thirty Duroc or Landrace breed boars were randomly selected for this study. The experimental group was fed a compound feed with added organic selenium and Oxynat 3D. The antioxidant status parameters of boar seminal plasma were evaluated using a biochemical analyzer and commercial Randox kits. The sperm chromatin structure assay (SCSA) using flow cytometry (FC) provided information about spermatozoa's DNA status. Additionally, the total number of motile spermatozoa and spermatozoa kinematic parameters were measured using the computer-assisted sperm analysis (CASA) system. The aim of this study was to improve the parameters of semen by combining two preparations that have a potential antioxidant effect, but also to establish the level of various antioxidant enzymes in native sperm. There was no statistically significant difference in total antioxidant capacity and glutathione peroxidase activity in the seminal plasma obtained from the experimental and control groups of boars. Regarding the superoxide dismutase activity, the research results showed a difference in the control group compared to the experimental one. Moreover, spermatozoa DNA fragmentation and the total number of motile spermatozoa showed statistically significant lower and higher values, respectively, in experimental compared to the control groups. The combination of these two preparations shows significantly enhanced vital parameters of semen. To the best of our knowledge, this study is the first in which the ejaculate parameters were examined after the application of a combination of these two antioxidant supplements.
该研究在塞尔维亚的一个商业养猪场进行。随机选择30头杜洛克或长白品种的公猪用于本研究。实验组饲喂添加了有机硒和Oxynat 3D的复合饲料。使用生化分析仪和兰道克斯商业试剂盒对公猪精液等离子体的抗氧化状态参数进行评估。使用流式细胞术（FC）的精子染色质结构分析（SCSA）提供了有关精子DNA状态的信息。此外，使用计算机辅助精子分析（CASA）系统测量活动精子的总数和精子运动学参数。本研究的目的是通过结合两种具有潜在抗氧化作用的制剂来改善精液参数，同时确定天然精子中各种抗氧化酶的水平。从实验组和对照组公猪获得的精液等离子体中，总抗氧化能力和谷胱甘肽过氧化物酶活性没有统计学上的显著差异。关于超氧化物歧化酶活性，研究结果显示对照组与实验组存在差异。此外，与对照组相比，实验组的精子DNA碎片化和活动精子总数分别显示出统计学上显著更低和更高的值。这两种制剂的组合显示精液的重要参数显著提高。据我们所知，本研究是首次在应用这两种抗氧化补充剂组合后检查射精参数。
