Calderón-Calderón Josué, Sevilla Francisco, Roldan Eduardo R S, Barquero Vinicio, Valverde Anthony
Animal Reproduction Laboratory, School of Agronomy, Costa Rica Institute of Technology, Alajuela, Costa Rica.
Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), Madrid, Spain.
Front Vet Sci. 2022 Jul 12;9:908763. doi: 10.3389/fvets.2022.908763. eCollection 2022.
Ejaculate quality can be regarded as multifactorial, with nutrition being a factor that could directly influence sperm parameters. The present study aimed to evaluate seminal quality associated with seasonal fat-soluble vitamin supplementation of boars. Seven sexually mature boars were randomly allotted to one of the three groups, and fed one of the three supplementary diets for 32 weeks: (1) control treatment (COD), without supplementation of fat-soluble vitamins, (2) treatment containing 100% fat-soluble vitamin supplementation administered intramuscularly, which was based on fat soluble vitamin supplementation (A, D3, E) (FVD1), and (3) treatment containing 50% of fat-soluble vitamin supplementation (FVD ). Semen was collected at 7-day intervals. Semen samples were analyzed to assess several sperm parameters using the Computer-Assisted Semen Analysis (CASA) ISASv1 system. Results showed that groups receiving FVD1 and FVD supplementation had an increased semen volume. The percentages of motile and progressively motile sperm were increased by FVD1 treatment. A statistically significant interaction between treatment and season was found in the percentage of motility and progressive motility ( < 0.05). Sperm concentrations showed significant differences ( < 0.05) between treatments. Velocity variables (VSL, VCL, and VAP) were higher ( < 0.05) in boars that received fat-soluble vitamin supplementation in comparison to controls receiving no supplementation. The FVD1 treatment presented spermatozoa with greater head size and more elongated heads ( < 0.05). Overall, the utilization of dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates. This highlights the importance of fat-soluble vitamin supplementation in sexually active boars.
射精质量可被视为多因素的，营养是一个可直接影响精子参数的因素。本研究旨在评估与公猪季节性补充脂溶性维生素相关的精液质量。七头性成熟公猪被随机分配到三组中的一组，并饲喂三种补充日粮之一，持续32周：（1）对照处理（COD），不补充脂溶性维生素；（2）基于脂溶性维生素补充剂（A、D3、E）进行肌肉注射的100%脂溶性维生素补充处理（FVD1）；（3）50%脂溶性维生素补充处理（FVD）。每隔7天采集一次精液。使用计算机辅助精液分析（CASA）ISASv1系统对精液样本进行分析，以评估多个精子参数。结果表明，接受FVD1和FVD补充的组精液体积增加。FVD1处理使活动精子和进行性活动精子的百分比增加。在活动率和进行性活动率的百分比方面，发现处理与季节之间存在统计学上的显著交互作用（P<0.05）。处理之间精子浓度存在显著差异（P<0.05）。与未补充的对照组相比，接受脂溶性维生素补充的公猪的速度变量（VSL、VCL和VAP）更高（P<0.05）。FVD1处理的精子头部尺寸更大且头部更细长（P<0.05）。总体而言，日粮补充脂溶性维生素显著改善了公猪射精的精液质量。这突出了在性活跃公猪中补充脂溶性维生素的重要性。
