在Wistar大鼠中使用荧光监测法对光卟啉进行药代动力学研究。

Pharmacokinetics of Photogem using fluorescence monitoring in Wistar rats.

作者信息

Melo C A S, Kurachi C, Grecco C, Sibata C H, Castro-e-Silva O, Bagnato V S

机构信息

Instituto de Física de São Carlos, University of Sao Paulo, Caixa Postal 369, 13560-970, Sao Carlos, SP, Brazil.

出版信息

J Photochem Photobiol B. 2004 Feb 20;73(3):183-8. doi: 10.1016/j.jphotobiol.2003.11.005.

DOI:10.1016/j.jphotobiol.2003.11.005

PMID:14975407

Abstract

In this study we investigated the pharmacokinetics of a hematoporphyrin derivative (Photogem) in Wistar rats using the fluorescence spectroscopy to evaluate the drug distribution in liver, kidney and skin tissues. The detection system is composed of a 532 nm exciting laser, a Y-type catheter for light delivery and collection, a monochromator and a computer for data acquisition. The analysis of the fluorescence spectra was based on the intensity of porphyrin emission bands from specific tissues of the investigated organ. A simple transport model is proposed to determine the accumulation and elimination times for each type of investigated tissue. The obtained results show the viability of the fluorescence spectroscopic technique for the drug concentration monitoring in different target tissues and related pharmacokinetics. These effects should be considered before any in vivo study of Photodynamic Therapy using Photogem.

摘要

在本研究中，我们使用荧光光谱法研究了血卟啉衍生物（光卟啉）在Wistar大鼠体内的药代动力学，以评估药物在肝脏、肾脏和皮肤组织中的分布。检测系统由一台532 nm激发激光器、一根用于光传输和收集的Y型导管、一个单色仪和一台用于数据采集的计算机组成。荧光光谱分析基于所研究器官特定组织中卟啉发射带的强度。提出了一个简单的转运模型来确定每种研究组织的积累和消除时间。所得结果表明，荧光光谱技术可用于监测不同靶组织中的药物浓度及相关药代动力学。在使用光卟啉进行光动力疗法的任何体内研究之前，都应考虑这些影响。