Floris Sarah, van der Goes Annette, Killestein Joep, Knol Dirk L, Barkhof Frederik, Polman Chris H, Dijkstra Christine D, de Vries Helga E, Meilof Jan F
Department of Molecular Cell Biology, VU Medical Center, P.O. Box 7057, 1007 MB, Amsterdam, The Netherlands.
J Neuroimmunol. 2004 Mar;148(1-2):172-7. doi: 10.1016/j.jneuroim.2003.11.005.
In active multiple sclerosis (MS) lesions macrophages expressing myeloid related protein (MRP) 8/14 are present. The aim of this study was to determine whether serum levels of MRP8/14 complexes are related to disease activity in MS. In a longitudinal study of 16 relapsing remitting (RR) MS patients that underwent monthly gadolinium diethylentriaminepenta acid (Gd-DTPA) magnetic resonance imaging (MRI), the relation between serum MRP8/14 levels and disease activity was investigated. Patients were participating in a monoclonal antibody study targeting a specific T cell population (Vbeta5.2/5.3+ T-cells). In time, within patients large variations in serum MRP8/14 levels were observed. Serum MRP8/14 levels were not related to changes in clinical disease activity or increase in Gd-DTPA lesion enhancement. Neither did comparison of active (>1 relapse in follow-up period) with inactive (0-1 relapse) MS patients reveal any differences in MRP8/14 levels. Therefore, we conclude that although MRP8/14 expression is a good histopathological marker for monocyte activation, serum levels of these proteins do not correlate with disease activity in RR MS.
在活动性多发性硬化（MS）病灶中存在表达髓样相关蛋白（MRP）8/14的巨噬细胞。本研究的目的是确定MRP8/14复合物的血清水平是否与MS的疾病活动相关。在一项对16例复发缓解型（RR）MS患者的纵向研究中，这些患者每月接受钆喷酸葡胺（Gd-DTPA）磁共振成像（MRI）检查，研究血清MRP8/14水平与疾病活动之间的关系。患者参与了一项针对特定T细胞群体（Vbeta5.2/5.3+ T细胞）的单克隆抗体研究。随着时间推移，观察到患者血清MRP8/14水平存在较大差异。血清MRP8/14水平与临床疾病活动的变化或Gd-DTPA病灶强化的增加无关。对活动期（随访期内>1次复发）与非活动期（0-1次复发）MS患者的比较也未发现MRP8/14水平有任何差异。因此，我们得出结论，尽管MRP8/14表达是单核细胞活化的良好组织病理学标志物，但这些蛋白的血清水平与RR MS的疾病活动无关。
