Kang Kihwa, Miyazaki Makoto, Ntambi James M, Pariza Michael W
Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
Biochem Biophys Res Commun. 2004 Mar 12;315(3):532-7. doi: 10.1016/j.bbrc.2004.01.087.
The trans-10,cis-12 isomer of conjugated linoleic acid (CLA) reduces body fat gain in animals and inhibits stearoyl-CoA desaturase (SCD) activity in 3T3-L1 adipocytes. To test whether CLA's body fat reduction is mediated by SCD1, wild-type and SCD1-null mice were fed diet supplemented with 0.2% trans-10,cis-12 (t10c12) CLA for 4 weeks. The t10c12 CLA-supplemented diet significantly reduced body fat mass in both wild type and SCD1-null mice. Similarly, t10c12 CLA diet decreased blood triglyceride and free fatty acid levels regardless of SCD1 genotypes. Mice fed t10c12 CLA exhibited increased mRNA expression of fatty acid synthase and uncoupling protein 2 in both genotypes. Taken together, the effects of t10c12 CLA on reduction of body fat gain, blood parameters, and mRNA expression in both SCD1-null mice and wild-type mice were similar, indicating that the anti-obesity effect of t10c12 CLA may be independent of the effects of this CLA isomer on SCD1 gene expression and enzyme activity.
共轭亚油酸（CLA）的反式-10，顺式-12异构体可减少动物体内脂肪增加，并抑制3T3-L1脂肪细胞中硬脂酰辅酶A去饱和酶（SCD）的活性。为了测试CLA减少体脂是否由SCD1介导，给野生型和SCD1基因敲除小鼠喂食添加0.2%反式-10，顺式-12（t10c12）CLA的饲料，持续4周。添加t10c12 CLA的饲料显著降低了野生型和SCD1基因敲除小鼠的体脂量。同样，无论SCD1基因型如何，t10c12 CLA饲料均可降低血液甘油三酯和游离脂肪酸水平。喂食t10c12 CLA的小鼠在两种基因型中均表现出脂肪酸合酶和解偶联蛋白2的mRNA表达增加。综上所述，t10c12 CLA对SCD1基因敲除小鼠和野生型小鼠体脂增加、血液参数及mRNA表达的影响相似，表明t10c12 CLA的抗肥胖作用可能独立于该CLA异构体对SCD1基因表达和酶活性的影响。
