Zhou Caibi, Hu Liuhong, Mu Ren, Mei Xin, Wu Xingli, Wang Chuanming, Zhou Xiaolu
College of Biological Science and Agriculture, Qiannan Normal University for Nationalities 5 Jianjiang Avenue Middle Section Duyun Guizhou 558000 China
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology Nakhon Ratchasima 30000 Thailand.
RSC Adv. 2022 Aug 26;12(37):24301-24310. doi: 10.1039/d2ra02831j. eCollection 2022 Aug 22.
This work aims to study the effect of compound green tea (CGT) on liver lipid metabolism in mice based on metabolomics of liquid chromatography-mass spectrometry (LC-MS), and preliminarily identify potential biomarkers and pathways of action by using a metabonomic network database to explore the lipid-lowering effect of CGT. In this study, forty mice were randomly divided into four groups: compound tea treatment group (DH), high-fat model control group (NK), normal control group (CK) and positive drug group (YK). After a month of different interventions, the mice were weighed and the blood lipid indexes were detected. In addition, differential liver metabolites were monitored by using LC-MS. The results showed that CGT and positive drug treatment were able to decrease body weight, liver coefficient, TC, TG and LDL levels of obese mice, while increasing HDL levels. Among the 110 compounds obtained, 54 metabolites were significantly altered in the four comparisons. More importantly, 15 remarkably downregulated metabolites involved in Lysopc 16:1, Lysopc 18:1, and Lysopc 18:2 were found in the DH group when the mice were treated with CGT; meanwhile, the positive drug Xuezhikang was able to significantly downregulate 14 compounds, including (±)18-HEPE, and 6 keto-PGF1α, compared with the NK group. Besides, KEGG enrichment analysis also revealed the important metabolic pathways, such as linoleic acid metabolism, Biosynthesis of unsaturated fatty acids, and α-linolenic acid metabolism, were related to fatty acid metabolism. These results suggested that CGT could regulate the lipid metabolism in the liver of hyperlipidemia mice, and may regulate 54 potential biomarkers in mice through a related metabolic pathway to make them return to a normal state and improve the disorder of lipid metabolism.
本研究旨在基于液相色谱 - 质谱联用（LC - MS）代谢组学研究复合绿茶（CGT）对小鼠肝脏脂质代谢的影响，并通过代谢组学网络数据库初步鉴定潜在生物标志物及作用途径，以探究CGT的降脂作用。本研究中，40只小鼠随机分为四组：复合茶治疗组（DH）、高脂模型对照组（NK）、正常对照组（CK）和阳性药物组（YK）。经过一个月的不同干预后，对小鼠称重并检测血脂指标。此外，采用LC - MS监测肝脏差异代谢物。结果表明，CGT和阳性药物治疗均能降低肥胖小鼠的体重、肝脏系数、总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平，同时提高高密度脂蛋白（HDL）水平。在所获得的110种化合物中，有54种代谢物在四项比较中发生了显著变化。更重要的是，当用CGT处理小鼠时，在DH组中发现15种参与溶血磷脂酰胆碱16:1、溶血磷脂酰胆碱18:1和溶血磷脂酰胆碱18:2的显著下调代谢物；同时，与NK组相比，阳性药物血脂康能够显著下调14种化合物，包括（±）18 - 羟基二十碳五烯酸（18 - HEPE）和6 - 酮 - 前列腺素F1α（6 keto - PGF1α）。此外，京都基因与基因组百科全书（KEGG）富集分析还揭示了重要的代谢途径，如亚油酸代谢、不饱和脂肪酸生物合成和α - 亚麻酸代谢，均与脂肪酸代谢相关。这些结果表明，CGT可调节高脂血症小鼠肝脏中的脂质代谢，并可能通过相关代谢途径调节小鼠体内54种潜在生物标志物，使其恢复正常状态，改善脂质代谢紊乱。
