Pojoga Luminita H, Moose Jana E, Hilderman Richard H
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634-0324, USA.
Biochem Biophys Res Commun. 2004 Mar 12;315(3):756-62. doi: 10.1016/j.bbrc.2004.01.121.
Adenylated dinucleotides (Ap(n)A) are regulatory molecules that control various cellular processes. A very likely intracellular target for Ap(4)A are enzymes that require ATP as either substrate or modulator. We report the results of new biochemical studies aimed at characterizing the Ap(4)A interaction with firefly luciferase, by using the luminometric and thin layer chromatography techniques. The data presented herein demonstrate that Ap(4)A is a noncompetitive inhibitor for the ATP-induced luminescence. These results together with our previous findings that Ap(4)A is a luciferase substrate [Nucleosides Nucleotides Nucleic Acids 23 (2004) in press.] support the notion that, similar to its interaction with P(2) receptors, Ap(4)A also has a dual interaction with luciferase. Other Ap(n)As (n = 2, 5, and 6) also inhibited the ATP-luciferase interaction. Since Ap(n)As may have similar interactions with other intracellular ATP-requiring enzymes, the study presented herein validates ulterior investigations of the Ap(n)A interaction with such enzymes, and opens the way to a better understanding of their intracellular roles.
腺苷化二核苷酸（Ap(n)A）是控制各种细胞过程的调节分子。Ap(4)A极有可能的细胞内靶点是那些需要ATP作为底物或调节剂的酶。我们报告了旨在通过使用发光法和薄层色谱技术来表征Ap(4)A与萤火虫荧光素酶相互作用的新生化研究结果。本文给出的数据表明，Ap(4)A是ATP诱导发光的非竞争性抑制剂。这些结果连同我们之前的发现，即Ap(4)A是荧光素酶的底物[《核苷、核苷酸与核酸》23（2004年）即将发表]，支持了这样一种观点，即与它和P(2)受体的相互作用类似，Ap(4)A与荧光素酶也存在双重相互作用。其他Ap(n)A（n = 2、5和6）也抑制了ATP - 荧光素酶的相互作用。由于Ap(n)A可能与其他细胞内需要ATP的酶存在类似的相互作用，本文的研究验证了对Ap(n)A与此类酶相互作用的进一步研究，并为更好地理解它们在细胞内的作用开辟了道路。
