Browning G, Wignall T A
Institute of Horticultural Research, East Malling, Maidstone, Kent ME19 6BJ, UK.
Tree Physiol. 1987 Sep;3(3):235-46. doi: 10.1093/treephys/3.3.235.
A technique is described for the rapid purification of indole-3-acetic acid (IAA) and abscisic acid (ABA) from small quantities of the basal trunk cambial region tissues of 34-year-old trees of Quercus robur L. Mass spectra of IAA and ABA obtained from the purified extracts provide the first reported unequivocal identification of endogenous IAA and ABA in the cambial region of a hardwood species. By use of the combined gas chromatography-mass spectrometry technique of multiple ion monitoring, with deuterated analogs as internal standards, it was shown that the levels of IAA in the cambial region tissues varied from ~1 to ~20 microg g(-1) and those of ABA from ~1 to ~5 microg g(-1) dry weight during the growing season. The high levels of IAA are similar to those found in the newly differentiating xylem tissues of softwoods.
本文描述了一种从34年生英国栎（Quercus robur L.）树干基部形成层区域的少量组织中快速纯化吲哚-3-乙酸（IAA）和脱落酸（ABA）的技术。从纯化提取物中获得的IAA和ABA的质谱图首次明确鉴定了硬木树种形成层区域内源性IAA和ABA。通过使用多离子监测的气相色谱-质谱联用技术，并以氘代类似物作为内标，结果表明，在生长季节，形成层区域组织中IAA的含量在约1至约20微克/克（-1）之间变化，ABA的含量在约1至约5微克/克（-1）干重之间变化。IAA的高水平与在软木新分化木质部组织中发现的水平相似。
