Department of Fisheries, Maritimes Forest Research Centre, P.O. Box 4000, E3B 5 P 7, Fredericton, N.B., Canada.
Planta. 1978 Jan;139(2):133-8. doi: 10.1007/BF00387138.
Indole-3-acetic acid (IAA) and abscisic acid (ABA) were identified by combined gas chromatography-mass spectrometry (GCMS) in fractions obtained by diffusion and extraction from bark peelings of Sitka spruce. A procedure is described for the quantitative analysis of IAA and ABA levels in the same extract using the GCMS technique of single-ion current monitoring. This procedure was used to measure the diffusible, free, and bound fractions of IAA and ABA in the cambial region of Sitka spruce throughout one year; the range in concentration for these fractions was 0.06-0.30, 0.46-3.85, and 0.04-0.20 μg/g oven-dry weight, respectively, for IAA, and 0-0.08, 0.03-2.21, and 0.13-0.66 μg/g oven-dry weight, respectively, for ABA. Movement in the cambial region was found to be polar for endogenous IAA and nonpolar for endogenous ABA. Recoveries of [(14)C]IAA internal standards showed that 73-99.5% of the IAA was lost during purification, and that there could be up to 5-fold differences in recovery between purifications, indicating that IAA loss shold be measured in quantitative analyses.
吲哚-3-乙酸（IAA）和脱落酸（ABA）通过扩散和从辐射松树皮中提取的馏分的气相色谱-质谱（GCMS）联合鉴定。描述了一种使用 GCMS 技术进行单离子电流监测的定量分析同一提取物中 IAA 和 ABA 水平的方法。该程序用于测量辐射松形成层中 IAA 和 ABA 的可扩散、游离和结合分数在一年中的变化；这些分数的浓度范围分别为 0.06-0.30、0.46-3.85 和 0.04-0.20μg/g 干重，0.00-0.08、0.03-2.21 和 0.13-0.66μg/g 干重，0.00-0.08、0.03-2.21 和 0.13-0.66μg/g 干重。发现内源 IAA 在形成层中的移动是极性的，而内源 ABA 是非极性的。[(14)C]IAA 内标回收率表明，在纯化过程中 IAA 损失了 73-99.5%，并且在不同的纯化过程中回收率可能有 5 倍的差异，这表明应该在定量分析中测量 IAA 的损失。
