Wu Y N, Vu N D, Wagner P D
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):697-700. doi: 10.1042/bj2850697.
Incubation of digitonin-permeabilized bovine chromaffin cells in the absence of Ca2+ results in a loss of both cytosolic proteins and Ca(2+)-dependent secretion. Addition of these leaked proteins prevents this loss of secretory activity. We have purified a protein from an extract of bovine adrenal medulla which can partially prevent this loss of Ca(2+)-dependent secretion. Antibody against this protein inhibited the ability of leaked chromaffin-cell proteins to prevent the loss of Ca(2+)-dependent secretion. Sequence analysis showed it to have sequence identity with bovine brain 14-3-3 protein. These results demonstrate that 14-3-3 protein makes a significant contribution to the ability of leaked chromaffin-cell proteins to maintain secretory activity.
在无Ca2+的情况下对洋地黄皂苷通透处理的牛嗜铬细胞进行孵育，会导致胞质蛋白和Ca(2+)依赖性分泌丧失。添加这些泄漏的蛋白可防止分泌活性的这种丧失。我们从牛肾上腺髓质提取物中纯化了一种蛋白，它可以部分防止Ca(2+)依赖性分泌的这种丧失。针对该蛋白的抗体抑制了嗜铬细胞泄漏蛋白防止Ca(2+)依赖性分泌丧失的能力。序列分析表明它与牛脑14-3-3蛋白具有序列同一性。这些结果表明，14-3-3蛋白对嗜铬细胞泄漏蛋白维持分泌活性的能力有重要贡献。
