Shapiro Howard M, Nebe-von-Caron Gerhard
The Center for Microbial Cytometry and Howard M. Shapiro, MD, PC, West Newton, NY, USA.
Methods Mol Biol. 2004;263:33-44. doi: 10.1385/1-59259-773-4:033.
The small size of bacteria makes some microbial constituents undetectable or measurable with only limited precision by flow cytometry. Bacteria may also behave differently from eukaryotes in terms of their interaction with dyes, drugs, and other reagents. It is therefore difficult to design multiparameter staining protocols that work, unmodified, across a wide range of bacterial species. This chapter describes reliable flow cytometric methods for assessment of the physiologic states of Gram-negative organisms, on the one hand, and Gram-positive organisms, on the other, based on measurement of membrane potential and membrane permeability. These techniques are useful in the assessment of effects of environmental conditions and antimicrobial agents on microorganisms.
细菌体积微小，使得一些微生物成分通过流式细胞术检测不到或只能以有限的精度进行测量。在与染料、药物和其他试剂的相互作用方面，细菌的行为也可能与真核生物不同。因此，很难设计出一种无需修改就能适用于广泛细菌种类的多参数染色方案。本章介绍了基于膜电位和膜通透性测量的可靠流式细胞术方法，一方面用于评估革兰氏阴性菌的生理状态，另一方面用于评估革兰氏阳性菌的生理状态。这些技术在评估环境条件和抗菌剂对微生物的影响方面很有用。
