Harry Perkins Institute of Medical Research, School of Medicine, Faculty of Health and Medical Sciences, the University of Western Australia, Nedlands, Western Australia, Australia.
Marshall Centre, School of Biomedical Sciences, Faculty of Health and Medical Sciences, the University of Western Australia, Nedlands, Western Australia, Australia.
Sci Rep. 2017 May 15;7(1):1903. doi: 10.1038/s41598-017-02009-3.
The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MIC) correspond closely with broth microdilution MIC (MIC, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.
全球范围内耐多药肺炎克雷伯菌的分布不断扩大，这就要求更快地进行抗菌药物敏感性检测（AST），以指导抗生素治疗。目前的 AST 依赖于在从临床标本中初步分离细菌后，对耐药性和敏感性进行耗时的区分。在这里，我们描述了一种流式细胞术工作流程，用于确定来自国际肺炎克雷伯菌分离株集（n = 48）中细菌细胞特征的碳青霉烯类药物敏感性，其中包含一系列碳青霉烯酶。我们的流式细胞术辅助药敏试验（FAST）方法结合了快速定性敏感/不敏感分类和定量 MIC 测量，在收到主要分离物后不久即可在单个过程中完成（分别为 54 分钟和 158 分钟）。定性 FAST 结果和 FAST 衍生的 MIC（MIC，马修相关系数 0.887）与肉汤微量稀释 MIC（MIC）非常吻合，与国际 AST 标准（ISO 200776-1；2006）一致，可用于更广泛的革兰氏阴性和革兰氏阳性细菌的快速抗菌药物敏感性测定。
