Samson Karen Thursday R, Chen Fen Hua, Miura Katsushi, Odajima Yasuhei, Iikura Yoji, Naval Rivas Maria, Minoguchi Kenji, Adachi Mitsuru
Department of Pediatrics, Showa University, School of Medicine, Tokyo, Japan.
Int Arch Allergy Immunol. 2004 Mar;133(3):225-32. doi: 10.1159/000076828. Epub 2004 Feb 17.
Characterization of seafood allergens is important to understand the immune response to these allergens. Moreover, a detailed comparison between atopic and nonatopic patients with adverse reactions to shrimp has never been reported.
Raw and boiled shrimp extracts were analyzed by immunoblotting using sera from 9 atopic and 7 nonatopic patients with a history of adverse reactions to shrimp, and 13 control subjects. Total IgE, specific IgE and skin prick tests (SPT) to shrimp were also investigated.
The level of specific IgE to shrimp was higher in atopic patients than nonatopic patients (p<0.05). Symptoms, SPT results and major allergens involved were similar in atopic and nonatopic patients. The 16.5-kD protein had the highest frequency of IgE binding followed by the 40-kD protein in these patients. Other minor IgE-binding proteins were observed at the 20-, 22-, 54-, 72-, 129- and 140-kD regions. Patients who had binding to the 16.5-kD protein had either positive (25% raw/31% cooked) or negative (13% raw/cooked) CAP-FEIA-RAST, while patients who recognized the 40-kD protein all had positive (31% raw/19% cooked) CAP-FEIA-RAST. All control subjects had negative immunoblots for these two proteins.
The 16.5-kD protein was the most frequent protein identified regardless of CAP-FEIA-RAST results, while the 40-kD protein was only present in patients with positive CAP-FEIA-RAST. Therefore, 16.5-kD protein may be an important allergen that is clinically relevant in both atopic and nonatopic patients with adverse reactions to shrimp even if it is not detected by the CAP-FEIA-RAST system.
对海鲜过敏原进行特性分析对于理解针对这些过敏原的免疫反应很重要。此外，从未有过关于对虾有不良反应的特应性和非特应性患者之间详细比较的报道。
使用来自9名有虾不良反应病史的特应性患者、7名有虾不良反应病史的非特应性患者以及13名对照受试者的血清，通过免疫印迹法分析生虾和煮虾提取物。还研究了针对虾的总IgE、特异性IgE和皮肤点刺试验（SPT）。
特应性患者中针对虾的特异性IgE水平高于非特应性患者（p<0.05）。特应性和非特应性患者的症状、SPT结果以及所涉及的主要过敏原相似。在这些患者中，16.5-kD蛋白的IgE结合频率最高，其次是40-kD蛋白。在20-、22-、54-、72-、129-和140-kD区域观察到其他次要的IgE结合蛋白。与16.5-kD蛋白结合的患者，其CAP-FEIA-RAST结果要么为阳性（生虾25%/熟虾31%），要么为阴性（生虾13%/熟虾），而识别40-kD蛋白的患者其CAP-FEIA-RAST均为阳性（生虾31%/熟虾19%）。所有对照受试者对这两种蛋白的免疫印迹均为阴性。
无论CAP-FEIA-RAST结果如何，16.5-kD蛋白都是最常鉴定出的蛋白，而40-kD蛋白仅存在于CAP-FEIA-RAST阳性的患者中。因此，16.5-kD蛋白可能是一种重要的过敏原，即使CAP-FEIA-RAST系统未检测到，它在对虾有不良反应的特应性和非特应性患者中均具有临床相关性。
