Aagaard Claus, Brock Inger, Olsen Anja, Ottenhoff Tom H M, Weldingh Karin, Andersen Peter
Department of Infectious Disease Immunology, Statens Serum Institute, Copenhagen, Denmark.
J Infect Dis. 2004 Mar 1;189(5):812-9. doi: 10.1086/381679. Epub 2004 Feb 13.
New tools are urgently needed for the detection of latent tuberculosis (TB). We evaluated the diagnostic potential of 2 novel Mycobacterium tuberculosis complex-specific candidate antigens (Rv2653 and Rv2654) and investigated T cell recognition during natural infection in humans and experimental infection in guinea pigs. Peripheral blood mononuclear cells stimulated with peptide pools covering the full length of Rv2654 induced interferon- gamma release in 10 of 19 patients with TB. Neither Rv2654 single peptides nor Rv2654 pools were recognized by bacille Calmette-Guerin-vaccinated donors. However, peptides from Rv2653 were recognized by both patients group. The cross-reactive epitope(s) in Rv2653 were located in a 36-amino acid stretch in the center of the molecule. Rv2654 also induced M. tuberculosis-specific skin-test responses in 3 of 4 aerosol-infected guinea pigs. Rv2654 is a strongly recognized T cell antigen that is highly specific for TB and has potential as a novel cell-mediated immunity-based TB diagnostic agent.
迫切需要用于检测潜伏性结核病（TB）的新工具。我们评估了两种新型结核分枝杆菌复合群特异性候选抗原（Rv2653和Rv2654）的诊断潜力，并研究了人类自然感染和豚鼠实验感染期间的T细胞识别情况。用覆盖Rv2654全长的肽池刺激外周血单个核细胞，在19例结核病患者中有10例诱导了干扰素-γ释放。卡介苗接种的供体既不识别Rv2654单个肽段，也不识别Rv2654肽池。然而，两组患者均识别来自Rv2653的肽段。Rv2653中的交叉反应表位位于分子中心36个氨基酸的区域。Rv2654在4只气溶胶感染的豚鼠中有3只诱导了结核分枝杆菌特异性皮肤试验反应。Rv2654是一种被强烈识别的T细胞抗原，对结核病具有高度特异性，有潜力作为一种基于细胞介导免疫的新型结核病诊断试剂。
