Trimmer James S, Rhodes Kenneth J
Department of Pharmacology, School of Medicine, University of California, Davis, California 95616-8635, USA.
Annu Rev Physiol. 2004;66:477-519. doi: 10.1146/annurev.physiol.66.032102.113328.
The intrinsic electrical properties of neurons are shaped in large part by the action of voltage-gated ion channels. Molecular cloning studies have revealed a large family of ion channel genes, many of which are expressed in mammalian brain. Much recent effort has focused on determining the contribution of the protein products of these genes to neuronal function. This requires knowledge of the abundance and distribution of the constituent subunits of the channels in specific mammalian central neurons. Here we review progress made in recent studies aimed at localizing specific ion channel subunits using in situ hybridization and immunohistochemistry. We then discuss the implications of these results in terms of neuronal physiology and neuronal mechanisms underlying the observed patterns of expression.
神经元的内在电特性在很大程度上由电压门控离子通道的作用所塑造。分子克隆研究揭示了一个庞大的离子通道基因家族，其中许多基因在哺乳动物大脑中表达。最近的许多工作都集中在确定这些基因的蛋白质产物对神经元功能的贡献上。这需要了解特定哺乳动物中枢神经元中通道组成亚基的丰度和分布。在这里，我们回顾了最近旨在使用原位杂交和免疫组织化学定位特定离子通道亚基的研究进展。然后，我们根据神经元生理学以及观察到的表达模式背后的神经元机制来讨论这些结果的意义。
