Carlone R L, Boulianne R P, Link A J
Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada.
Biochem Cell Biol. 1992 May;70(5):285-90. doi: 10.1139/o92-045.
We have used high resolution two-dimensional gel electrophoresis to identify and characterize proteins that may represent products of genes involved in establishing positional information along the proximal-distal axis of the regenerating forelimb of the newt Notophthalmus viridescens. At least 24 proteins have been found whose synthesis and (or) abundance is increased in proximal (midstylopodial) regenerates relative to midzeugopodial (distal) regenerates at either of two regeneration stages, the early dedifferentiation and moderate bud stages. Four of these same proteins show an axial asymmetry at both stages. Ten distal-specific proteins were also identified, although only one was common to both stages. More significantly, 6 of these 34 proteins (molecular masses of 73, 73, 51.5, 44.0, 19.5, and 16.5 kilodaltons and isoelectric points of 6.70, 6.74, 6.0, 6.05, 5.9, and 6.98, respectively) are regulated to proximal levels by treatment of distal regenerates with retinoic acid (RA) at both stages. An additional five are proximalized by RA at only one regeneration stage. Since the effect of RA is to proximalize positional information in blastema cells, these 11 proteins represent gene products that could be involved in a biochemical cascade leading to the establishment of positional information in the regenerating limb along this axis.
我们利用高分辨率二维凝胶电泳来鉴定和表征可能代表参与沿绿螈Notophthalmus viridescens再生前肢近端-远端轴建立位置信息的基因产物的蛋白质。已发现至少24种蛋白质，在两个再生阶段（早期去分化阶段和适度芽阶段）中的任一阶段，相对于中zeugopodial（远端）再生体，其在近端（中stylopodial）再生体中的合成和（或）丰度增加。其中四种相同的蛋白质在两个阶段均表现出轴向不对称性。还鉴定出了十种远端特异性蛋白质，尽管两个阶段仅有一种是共同的。更重要的是，这34种蛋白质中的6种（分子量分别为73、73、51.5、44.0、19.5和16.5千道尔顿，等电点分别为6.70、6.74、6.0、6.05、5.9和6.98）在两个阶段通过用视黄酸（RA）处理远端再生体而被调节至近端水平。另外五种仅在一个再生阶段被RA近端化。由于RA的作用是使芽基细胞中的位置信息近端化，因此这11种蛋白质代表可能参与导致沿该轴在再生肢体中建立位置信息的生化级联反应的基因产物。
