Crawford K, Stocum D L
University of Illinois, Department of Cell and Structural Biology, Urbana 61801.
Development. 1988 Apr;102(4):687-98. doi: 10.1242/dev.102.4.687.
An assay that detects position-related differences in affinity of axolotl regeneration blastema cells in vivo was used to test whether retinoic acid, which proximalizes regenerate pattern, simultaneously proximalizes blastema cell affinity. The assay involved autografting or homografting late bud forelimb blastomas derived from the wrist, elbow or midupper arm levels to the dorsal surface of the blastema-stump junction of an ipsilateral, medium-bud-stage hindlimb regenerating from the midthigh level. The grafted blastemas consistently displaced to their corresponding levels on the proximodistal axis of the host regenerate, indicating the existence of level-specific differences in blastema cell affinity. Retinoic acid proximalized the pattern of donor forelimb regenerates to the level of the girdle and abolished their displacement behaviour on untreated host hindlimbs. Conversely, untreated forelimb donor blastemas displaced distally to their corresponding levels on host ankle regenerates, that had been proximalized to the level of the girdle by retinoic acid. These results indicate that positional memory in regenerating limbs is directly related to blastema cell affinity, and that very similar or identical sets of level-specific affinity properties are shared by forelimb and hindlimb cells.
一种用于检测蝾螈再生芽基细胞在体内亲和力位置相关差异的测定方法，被用于测试视黄酸（它能使再生模式近端化）是否同时使芽基细胞亲和力近端化。该测定方法包括将源自腕部、肘部或上臂中部水平的晚期芽前肢芽瘤自体移植或同种移植到同侧、从中大腿水平再生的中芽期后肢芽基-残端连接处的背侧表面。移植的芽基始终会在宿主再生的近端-远端轴上移位到其相应水平，这表明芽基细胞亲和力存在水平特异性差异。视黄酸使供体前肢再生模式近端化到腰带水平，并消除了它们在未处理的宿主后肢上的移位行为。相反，未处理的前肢供体芽基会向远端移位到宿主踝关节再生的相应水平，而宿主踝关节再生已通过视黄酸近端化到腰带水平。这些结果表明，再生肢体中的位置记忆与芽基细胞亲和力直接相关，并且前肢和后肢细胞具有非常相似或相同的一组水平特异性亲和力特性。
