Pérez-Gil J, Tucker J, Simatos G, Keough K M
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Biochem Cell Biol. 1992 May;70(5):332-8. doi: 10.1139/o92-051.
Hydrophobic pulmonary surfactant protein enriched in SP-C has been mixed in amounts up to 10% by weight with various phospholipids. The lipids used were dipalmitoyl phosphatidylcholine (DPPC), or DPPC plus unsaturated phosphatidylglycerol (PG), or phosphatidylinositol (PI) in molar ratios of 9:1 and 7:3. The protein enhanced the rate and extent of adsorption of each lipid preparation into the air-water interface, and its respreading after compression on a surface balance. Maximum surface pressures attained on compression of monolayers of mixtures of lipids were slightly higher in the presence of protein. The effects on rate and extent of adsorption were proportional to the amount of protein present. Mixtures containing 30 mol% PG or PI adsorbed more readily into the interface than those containing 10% acidic lipid or DPPC alone. Mixtures containing 30% PI were slightly more rapidly adsorbed than those containing 30% PG. The results suggest that mixtures of DPPC with either acidic lipid in the presence of surfactant protein could be effective in artificial surfactants.
富含表面活性蛋白C的疏水性肺表面活性剂已与各种磷脂按重量比高达10%进行混合。所使用的脂质为二棕榈酰磷脂酰胆碱（DPPC），或DPPC加不饱和磷脂酰甘油（PG），或摩尔比为9:1和7:3的磷脂酰肌醇（PI）。该蛋白提高了每种脂质制剂吸附到气-水界面的速率和程度，以及在表面天平上压缩后其再铺展的能力。在存在蛋白的情况下，脂质混合物单层压缩时达到的最大表面压力略高。对吸附速率和程度的影响与存在的蛋白量成正比。含有30摩尔%PG或PI的混合物比含有10%酸性脂质或单独的DPPC的混合物更容易吸附到界面中。含有30%PI的混合物比含有30%PG的混合物吸附得稍快一些。结果表明，在表面活性剂蛋白存在下，DPPC与酸性脂质的混合物可有效用于人工表面活性剂。
