Andéol Y, Méchali M, Hourdry J
CNRS Institut Jacques Monod, Paris, France.
Mol Reprod Dev. 1992 Jul;32(3):187-95. doi: 10.1002/mrd.1080320302.
The expression of the ras protooncogene was investigated in Xenopus laevis, throughout development, by in situ hybridization using a 35S-labelled antisense RNA probe. During oogenesis, the ras RNA was strongly expressed in the cytoplasm of previtellogenic oocytes and further diluted between yolk platelets; no specific localization of transcripts was observed. The signal density was particularly weak over embryo sections until the tailbud stage. On the other hand, a high level of ras RNA expression was detected on sections through the young tadpoles. An intense labelling was observed in several areas, including the branchial apparatus, gut, somites, nervous system, and lens. It is noteworthy that the heterogeneity of labelling increases as tadpoles grow older. Together, these results are discussed in relation to cellular events appearing throughout the early development.
利用35S标记的反义RNA探针，通过原位杂交技术，研究了非洲爪蟾整个发育过程中ras原癌基因的表达情况。在卵子发生过程中，ras RNA在前卵黄生成期卵母细胞的细胞质中强烈表达，并在卵黄小板之间进一步稀释；未观察到转录本的特异性定位。在尾芽期之前，胚胎切片上的信号密度特别弱。另一方面，在幼体蝌蚪的切片上检测到高水平的ras RNA表达。在几个区域观察到强烈的标记，包括鳃器、肠道、体节、神经系统和晶状体。值得注意的是，随着蝌蚪年龄的增长，标记的异质性增加。总之，结合整个早期发育过程中出现的细胞事件对这些结果进行了讨论。
