Baum E Z, Bebernitz G A
Molecular Biology Section, Lederle Laboratories, American Cyanamid Company, Pearl River, NY 10965.
Oncogene. 1990 May;5(5):763-7.
A Xenopus laevis homolog of mammalian Kirsten-ras has been isolated from an oocyte cDNA library. This ras clone has been used to examine the genomic representation and expression of ras in oocytes and embryos. The Xenopus homolog of K-ras is a low-copy gene encoding a 2.6 kb mRNA, which is present throughout oogenesis and embryonic development. DNA sequence analysis indicates that Xenopus and mammalian K-ras 2B are highly conserved at the mRNA level (82%) and encode nearly identical proteins. It should now be feasible to address the function of endogenous ras oncogene in the well-characterized Xenopus developmental system.
已从非洲爪蟾卵母细胞cDNA文库中分离出一种与哺乳动物 Kirsten-ras 同源的非洲爪蟾基因。该ras克隆已用于检测卵母细胞和胚胎中ras的基因组表现形式和表达情况。K-ras的非洲爪蟾同源物是一个低拷贝基因，编码一种2.6 kb的mRNA，在整个卵子发生和胚胎发育过程中都存在。DNA序列分析表明，非洲爪蟾和哺乳动物的K-ras 2B在mRNA水平上高度保守（82%），并编码几乎相同的蛋白质。现在，在特征明确的非洲爪蟾发育系统中研究内源性ras癌基因的功能应该是可行的。
