Prather R S, Schatten G
Department of Zoology, University of Wisconsin-Madison 65211.
Mol Reprod Dev. 1992 Jul;32(3):203-8. doi: 10.1002/mrd.1080320304.
The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize nuclear matrix antigens in germinal vesicle oocytes, metaphase II oocytes, zygotes, two-cell-stage embryos, and eight-cell stage embryos. All antibodies reacted with the nuclei of germinal vesicle oocytes, and two- and eight-cell-stage embryos; however, only P1 and lamin B were present at the pronuclear stage. In eggs collected at the pronuclear stage and cultured to the late two-cell stage in the presence of alpha-amanitin, the matrix morphology was altered for Pl1 and Pl2. alpha-Amanitin had no affect on the distribution of P1 or lamin B antigens. If alpha-amanitin was added 2 hr after cleavage to the two-cell stage, the normal staining pattern of Pl2 was retained. These results suggest that the presence of specific components of an internal matrix is correlated with normal genomic activity.
核基质被认为与DNA组织、DNA复制、RNA合成及RNA加工有关。我们研究了小鼠胚胎发育早期核基质抗原的存在情况。利用针对外周和内部抗原（P1、Pl1、Pl2和核纤层蛋白B）的抗体，对生发泡卵母细胞、减数第二次分裂中期卵母细胞、受精卵、二细胞期胚胎和八细胞期胚胎中的核基质抗原进行免疫定位。所有抗体均与生发泡卵母细胞以及二细胞期和八细胞期胚胎的细胞核发生反应；然而，仅P1和核纤层蛋白B在原核期存在。在原核期收集的卵中，于α-鹅膏蕈碱存在的情况下培养至二细胞晚期，Pl1和Pl2的基质形态发生改变。α-鹅膏蕈碱对P1或核纤层蛋白B抗原的分布没有影响。如果在二细胞期分裂后2小时添加α-鹅膏蕈碱，则Pl2的正常染色模式得以保留。这些结果表明，内部基质特定成分的存在与正常基因组活性相关。
