Prather R S, Kubiak J, Maul G G, First N L, Schatten G
Department of Zoology, University of Wisconsin, Madison 53706.
J Exp Zool. 1991 Jan;257(1):110-4. doi: 10.1002/jez.1402570114.
The cytoplasmic regulation of changes of nuclear lamin antigens was examined by transferring 16-cell stage blastomeres into mouse oocytes. Sixteen-cell stage blastomeres were transferred to either pronuclear eggs, enucleated pronuclear eggs or metaphase II oocytes, which were subsequently activated. Pronuclei react with a monoclonal antibody to A/C lamins (J9), whereas nuclei from 16-cell stage blastomeres do not react with J9. However, after transfer of 16-cell stage nuclei to activated metaphase II oocytes, the transferred nuclei acquire the antigen. This is in contrast to 16-cell nuclei that were transferred to intact or enucleated pronuclear eggs; i.e., the nuclei only faintly acquired the A/C epitope. These results suggest that the developmental stage of the cytoplasm regulates the exposure of nuclear lamina epitopes, perhaps by limiting the supply of lamin A/C in the oocyte or because nuclear lamina assembly can only occur at the telophase transition. Furthermore, it appears that there is some exchange of the A/C epitope between (pro)nuclei within the same cell but that the majority of the A/C lamin epitope can be removed from a cell with (pro)nuclear removal.
通过将16细胞期的卵裂球转移到小鼠卵母细胞中，研究了核纤层抗原变化的细胞质调控。将16细胞期的卵裂球转移到原核卵、去核原核卵或中期II卵母细胞中，随后对其进行激活。原核对抗A/C型核纤层蛋白的单克隆抗体（J9）有反应，而16细胞期卵裂球的细胞核对J9无反应。然而，将16细胞期的细胞核转移到激活的中期II卵母细胞后，转移的细胞核获得了该抗原。这与转移到完整或去核原核卵中的16细胞期细胞核形成对比；即，这些细胞核仅微弱地获得A/C表位。这些结果表明，细胞质的发育阶段可能通过限制卵母细胞中核纤层蛋白A/C的供应，或者因为核纤层组装只能在末期转变时发生，从而调节核纤层表位的暴露。此外，似乎同一细胞内的（原）核之间存在一些A/C表位的交换，但大部分A/C型核纤层蛋白表位可随着（原）核的去除而从细胞中去除。
