Friedman B, Scherer S S, Rudge J S, Helgren M, Morrisey D, McClain J, Wang D Y, Wiegand S J, Furth M E, Lindsay R M
Regeneron Pharmaceuticals Inc. Tarrytown, New York 10591.
Neuron. 1992 Aug;9(2):295-305. doi: 10.1016/0896-6273(92)90168-d.
Adult rat sciatic nerve is known to express high levels of ciliary neurotrophic factor (CNTF) mRNA and protein. Here we examine the cellular localization of CNTF protein and mRNA in peripheral nerve and the regulation of CNTF expression by peripheral axons. In intact nerve, CNTF immunoreactivity is found predominantly in the cytoplasm of myelin-related Schwann cells. After axotomy, CNTF immunoreactivity and mRNA levels fall dramatically and do not recover unless axons regenerate. This behavior is similar to the pattern of myelin gene expression in these nerves. We conclude that the expression of CNTF in Schwann cells depends on axon-Schwann cell interactions.
已知成年大鼠坐骨神经表达高水平的睫状神经营养因子（CNTF）mRNA和蛋白质。在此，我们研究了CNTF蛋白和mRNA在周围神经中的细胞定位以及周围轴突对CNTF表达的调节。在完整神经中，CNTF免疫反应性主要存在于与髓鞘相关的施万细胞的细胞质中。轴突切断后，CNTF免疫反应性和mRNA水平急剧下降，除非轴突再生，否则不会恢复。这种行为与这些神经中髓鞘基因表达的模式相似。我们得出结论，施万细胞中CNTF的表达取决于轴突与施万细胞的相互作用。
