Al Abri Rashid, Kolethekkat Arif Ali, Kelleher Michael O, Myles Lynn M, Glasby Michael A
ENT Division, Surgery Department, College of Medicine & Health Sciences, Sultan Qaboos University, P.O. Box 38, Al Khod 123, Muscat, Sultanate of Oman.
Department of Neurosurgery, Beaumont Hospital, Dublin, Ireland.
Oman Med J. 2014 May;29(3):208-13. doi: 10.5001/omj.2014.51.
to determine whether the administration of Ciliary Neurotrophic Factor (CNTF) at the site of repaired facial nerve enhances regeneration in the adult sheep model.
Ten adult sheep were divided into 2 groups: control and study group (CNTF group). In the CNTF group, the buccal branch of the facial nerve was transected and then repaired by epineural sutures. CNTF was injected over the left depressor labii maxillaris muscle in the vicinity of the transected and repaired nerve for 28 days under local anesthesia. In the CNTF group, the sheep were again anesthetized after nine months and the site of facial nerve repair was exposed. Detailed electrophysiological, tension experiments and morphometric studies were carried out and then analyzed statistically.
The skin CV min, refractory period, Jitter and tension parameters were marginally raised in the CNTF group than the control but the difference was statistically insignificant between the two groups. Morphometric indices also did not show any significant changes in the CNTF group.
CNTF has no profound effect on neuronal regeneration of adult sheep animal model.
CNTF; Neurtrophic factors; Sheep; Facial nerve; Regeneration.
确定在成年绵羊模型中，在修复的面神经部位给予睫状神经营养因子（CNTF）是否能促进神经再生。
将10只成年绵羊分为2组：对照组和研究组（CNTF组）。在CNTF组中，切断面神经颊支，然后采用神经外膜缝合进行修复。在局部麻醉下，于切断并修复的神经附近的左侧降口角肌上注射CNTF，持续28天。9个月后，再次对CNTF组绵羊进行麻醉，暴露面神经修复部位。进行详细的电生理、张力实验和形态学研究，然后进行统计学分析。
CNTF组的皮肤复合肌肉动作电位最小波幅、不应期、肌纤维震颤和张力参数略高于对照组，但两组之间差异无统计学意义。形态学指标在CNTF组也未显示出任何显著变化。
CNTF对成年绵羊动物模型的神经元再生没有显著影响。
CNTF；神经营养因子；绵羊；面神经；再生。
