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来自存档处理过的粘性提取器的参考DNA的一个可能来源。

A possible source of reference DNA from archived treated adhesive lifters.

作者信息

Zamir Ashira, Oz Carla, Wolf Ehud, Vinokurov Asya, Glattstein Baruch

机构信息

Forensic Biology Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National Headquarters, Jerusalem, Israel.

出版信息

J Forensic Sci. 2004 Jan;49(1):68-70.

PMID:14979346
Abstract

During the course of a double murder trial, it became apparent that the two adhesive lifters from the two cadavers had been mislabeled before being presented in court. The question was raised whether DNA testing from the biological material remaining attached to the lifters could resolve this mix-up. In fatal shooting cases where a bullet has been fired through a body surface, an adhesive lifter is applied directly to the entrance wound. The total nitrite residues, as well as biological material surrounding the wound (blood, hair, tissue) are transferred to the adhesive lifter. The nitrite residues are used for estimating firing distance. In a worst-case scenario, the biological material on the lifter may be the only remaining reference material from a victim. In this paper, we examined whether the biological material retrieved from adhesive lifters could be used for DNA typing after the lifters had been treated for GSR pattern. In as much as the biological material found on the lifters can be typed and profiled following physical and chemical treatment, we submit that archived adhesive lifters can be used as a future source of reference DNA from cadavers where no other sample is available.

摘要

在一场双重谋杀案审判过程中,很明显,来自两名受害者尸体的两个粘性提取器在提交法庭之前就被错误标记了。于是有人提出疑问,对仍附着在提取器上的生物材料进行DNA检测是否能解决这一混淆问题。在致命枪击案件中,若子弹穿过身体表面,粘性提取器会直接应用于伤口入口处。伤口周围的总亚硝酸盐残留以及生物材料(血液、毛发、组织)会转移到粘性提取器上。亚硝酸盐残留用于估计射击距离。在最坏的情况下,提取器上的生物材料可能是受害者仅存的参考材料。在本文中,我们研究了从粘性提取器上获取的生物材料在经过枪支射击残留物(GSR)模式处理后是否可用于DNA分型。鉴于经过物理和化学处理后,提取器上发现的生物材料能够进行分型和分析,我们认为,在没有其他样本可用的情况下,存档的粘性提取器可作为未来从尸体获取参考DNA的来源。

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