Hamer-Barrera Roberto, Godínez Delfino, Enríquez V Idalia, Vaca-Pacheco Sergio, Martinez-Zúñiga Rodrigo, Talamás-Rohana Patricia, Suárez-Güemez Francisco, de la Garza Mireya
Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN, Ap. postal 14-740, México DF 07000, México.
Can J Vet Res. 2004 Jan;68(1):33-41.
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetra-acetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract.
对猪胸膜肺炎放线杆菌血清1型这种猪病原体粘附猪、大鼠和人的颊上皮细胞（BEC）的能力进行了研究。粘附细菌数量最多的是猪BEC。这种结合能力受到加热、极端pH值、用十二烷基硫酸钠、乙二胺四乙酸或高碘酸盐处理以及蛋白水解的影响，这表明细胞表面糖蛋白参与了粘附，并且粘附主要基于离子相互作用。甘露糖和猪纤连蛋白可能在这种相互作用中起直接作用。自然感染猪的恢复期血清可抑制粘附。细菌致病性以及宿主特异性与粘附猪BEC的能力之间存在相关性。粘附猪BEC为体外研究猪胸膜肺炎放线杆菌和猪呼吸道其他病原体的粘附提供了一种便捷方法。
