史蒂文秋水仙（Colchicum steveni）和耶路撒冷秋水仙（C. hierosolymitanum）（秋水仙科）中秋水仙碱的测定：两种分析方法的比较

Determination of colchicine in Colchicum steveni and C. hierosolymitanum (Colchicaceae): comparison between two analytical methods.

作者信息

Alali Feras, Tawaha Khaled, Qasaymeh Rana M

机构信息

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.

出版信息

Phytochem Anal. 2004 Jan-Feb;15(1):27-9. doi: 10.1002/pca.738.

DOI:10.1002/pca.738

PMID:14979523

Abstract

The amounts of colchicine present in two Jordanian species of Colchicum, namely, C. steveni Kunth and C. hierosolymitanum Feibrun (Colchicaceae), have been determined. An HPLC-UV (photodiode array) method employing gradient elution was developed and the results compared with those obtained using a simple TLC-spectrophotometric method. The levels of colchicine as measured by these methods were not significantly different (p < 0.05) indicating that the spectrophotometric method is an acceptable alternative to HPLC. With respect to C. steveni, the leaves contained the largest amount of colchicine (0.204/100 g), whilst in C. hierosolymitanum corms showed the highest colchicine content (0.126/100 g). As a source of colchicine, the two investigated species showed levels comparable with those found in C. autumnale, the traditional source of colchicine.

摘要

已测定了约旦两种秋水仙属植物，即史蒂文秋水仙（Colchicum steveni Kunth）和耶路撒冷秋水仙（Colchicum hierosolymitanum Feibrun）（秋水仙科）中的秋水仙碱含量。开发了一种采用梯度洗脱的高效液相色谱 - 紫外（光电二极管阵列）方法，并将结果与使用简单薄层色谱 - 分光光度法获得的结果进行比较。通过这些方法测得的秋水仙碱水平没有显著差异（p < 0.05），表明分光光度法是高效液相色谱的一种可接受的替代方法。对于史蒂文秋水仙，叶片中秋水仙碱含量最高（0.204/100克），而在耶路撒冷秋水仙中，球茎的秋水仙碱含量最高（0.126/100克）。作为秋水仙碱的来源，这两种被研究的物种所显示的含量水平与秋水仙碱的传统来源秋番红花（Colchicum autumnale）中的含量相当。