Misra Ankita, Shukla Pushpendra Kumar, Kumar Bhanu, Chand Jai, Kushwaha Poonam, Khalid Md, Singh Rawat Ajay Kumar, Srivastava Sharad
Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Department of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India.
Pharmacogn Mag. 2017 Oct;13(Suppl 3):S700-S705. doi: 10.4103/pm.pm_576_16. Epub 2017 Oct 11.
L. (Colchicaceae) is used as adjuvant therapy in gout for its potential antimitotic activity due to high colchicine(s) alkaloids.
This study aimed to develop an easy, cheap, precise, and accurate high-performance thin-layer chromatographic (HPTLC) validated method for simultaneous quantification of bioactive alkaloids (colchicine and gloriosine) in L. and to identify its elite chemotype(s) from Sikkim Himalayas (India).
The HPTLC chromatographic method was developed using mobile phase of chloroform: acetone: diethyl amine (5:4:1) at λ of 350 nm.
Five germplasms were collected from targeted region, and on morpho-anatomical inspection, no significant variation was observed among them. Quantification data reveal that content of colchicine (: 0.72) and gloriosine (: 0.61) varies from 0.035%-0.150% to 0.006%-0.032% (dry wt. basis). Linearity of method was obtained in the concentration range of 100-400 ng/spot of marker(s), exhibiting regression coefficient of 0.9987 (colchicine) and 0.9983 (gloriosine) with optimum recovery of 97.79 ± 3.86 and 100.023% ± 0.01%, respectively. Limit of detection and limit of quantification were analyzed, respectively, as 6.245, 18.926 and 8.024, 24.316 (ng). Two germplasms, namely NBG-27 and NBG-26, were found to be elite chemotype of both the markers.
The developed method is validated in terms of accuracy, recovery, and precision studies as per the ICH guidelines (2005) and can be adopted for the simultaneous quantification of colchicine and gloriosine in phytopharmaceuticals. In addition, this study is relevant to explore the chemotypic variability in metabolite content for commercial and medicinal purposes.
An easy, cheap, precise, and accurate high performance thin layer chromatographic (HPTLC) validated method for simultaneous quantification of bioactive alkaloids (colchicine and gloriosine) in G. superba L.Five germplasms were collected from targeted region, and on morpho anatomical inspection, no significant variation was observed among themQuantification data reveal that content of colchicine (Rf: 0.72) and gloriosine (Rf: 0.61) varies from 0.035%-0.150% to 0.006%-0.032% (dry wt. basis)Two germplasms, namely NBG 27 and NBG 26, were found to be elite chemotype of both the markers.
由于秋水仙碱类生物碱含量高，丽江山慈菇（秋水仙科）因其潜在的抗有丝分裂活性而被用作痛风的辅助治疗药物。
本研究旨在开发一种简便、廉价、精确且准确的经过验证的高效薄层色谱（HPTLC）方法，用于同时定量丽江山慈菇中的生物活性生物碱（秋水仙碱和光慈菇辛），并从印度锡金喜马拉雅地区鉴定其优良化学型。
采用氯仿∶丙酮∶二乙胺（5∶4∶1）为流动相，在350 nm波长下建立HPTLC色谱法。
从目标区域收集了5个种质，经形态解剖学检查，它们之间未观察到显著差异。定量数据显示，秋水仙碱（比移值：0.72）和光慈菇辛（比移值：0.61）的含量在0.035% - 0.150%至0.006% - 0.032%（干重基础）之间变化。该方法在标记物浓度范围为100 - 400 ng/斑点时具有线性关系，秋水仙碱的回归系数为0.9987，光慈菇辛的回归系数为0.9983，最佳回收率分别为97.79 ± 3.86和100.023% ± 0.01%。分别分析了检测限和定量限，结果为6.245、18.926和8.024、24.316（ng）。发现两个种质，即NBG - 27和NBG - 26，是两种标记物的优良化学型。
根据ICH指南（2005年），所开发的方法在准确性、回收率和精密度研究方面得到了验证，可用于同时定量植物药中秋水仙碱和光慈菇辛。此外，本研究对于探索用于商业和药用目的的代谢物含量的化学型变异性具有重要意义。
一种简便、廉价、精确且准确的经过验证的高效薄层色谱（HPTLC）方法，用于同时定量丽江山慈菇中的生物活性生物碱（秋水仙碱和光慈菇辛）。从目标区域收集了5个种质，经形态解剖学检查，它们之间未观察到显著差异。定量数据显示，秋水仙碱（比移值：0.72）和光慈菇辛（比移值：0.61）的含量在0.035% - 0.150%至0.006% - 0.032%（干重基础）之间变化。发现两个种质，即NBG - 27和NBG - 26，是两种标记物的优良化学型。
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