约旦藏红花（秋水仙科）——史蒂文氏秋水仙的植物化学研究及细胞毒性评估

Phytochemical study and cytotoxicity evaluation of Colchicum stevenii Kunth (Colchicaceae): a Jordanian meadow saffron.

作者信息

Al-Mahmoud Mohsen S, Alali Feras Q, Tawaha Khaled, Qasaymeh Rana M

机构信息

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, PO Box 3030, Irbid 22110, Jordan.

出版信息

Nat Prod Res. 2006 Feb;20(2):153-60. doi: 10.1080/14786410500046224.

DOI:10.1080/14786410500046224

PMID:16319009

Abstract

Isolation, characterization, and biological evaluation of active components of Colchicum stevenii Kunth (Colchicaceae) are described. Colchicum stevenii is an unexplored Jordanian specie with toxic reputation. Directed by brine shrimp lethality test (BST), methanolic extraction, liquid-liquid partition, preparative TLC, and semi-preparative HPLC, it resulted in the isolation of six cytotoxic compounds. The compounds, reported for the first time from this specie, are: (-)-colchicine (1), 2-demethyl-(-)-colchicine (2), (-)-cornigerine (3), beta-lumicolchicine (4), (-)-isoandrocymbine (5) and (-)-O-methylandrocymbine (6). A new, in-house developed, acidic-based reverse-phase gradient semi-preparative HPLC method for the separation of colchisides is presented here. Structural elucidation was based on spectroscopic techniques principally; 1H-NMR and low resolution EIMS. Based on BST results, reported as LC50 values in microg mL(-1) (ppm) with 95% confidence intervals, (-)-colchicine (2.5 ppm) and (-)-cornigerine (2.7 ppm) were the most potent.

摘要

本文描述了对史蒂文氏秋水仙（秋水仙科）活性成分的分离、表征及生物学评价。史蒂文氏秋水仙是约旦一种尚未被研究的植物，素有毒性之名。通过卤虫致死试验（BST）指导，进行甲醇提取、液 - 液分配、制备薄层层析和半制备高效液相色谱，最终分离出六种具有细胞毒性的化合物。这些化合物首次从该植物中分离得到，分别为：(-)-秋水仙碱（1）、2 - 去甲基 - (-)-秋水仙碱（2）、(-)-考尼吉林（3）、β - 光秋水仙碱（4）、(-)-异雄麻碱（5）和(-)-O - 甲基雄麻碱（6）。本文介绍了一种新的、内部开发的基于酸性的反相梯度半制备高效液相色谱法用于秋水仙苷的分离。结构解析主要基于光谱技术，即1H - NMR和低分辨电子轰击质谱（EIMS）。根据BST结果，以微克每毫升（ppm）表示的LC50值及95%置信区间显示，(-)-秋水仙碱（2.5 ppm）和(-)-考尼吉林（2.7 ppm）的细胞毒性最强。