Bennetau-Pelissero C, Latonnelle K Gontier, Lamothe V, Shinkaruk-Poix S, Kaushik S J
Micronutriments, Reproduction and Health Unit, ENITA de Bordeaux, 1 cours du General de Gaulle, 33175 Gradignan, France.
Phytochem Anal. 2004 Jan-Feb;15(1):40-5. doi: 10.1002/pca.741.
The use of in vitro trout hepatocyte cultures is shown to provide a simple and effective way to screen plant and food products for oestrogenic activity. The relative oestrogenic activities of 0.1 g each of extracts of phytosterol, soy isoflavone, red clover, kudzu and soybean extracts were determined using this assay and found to be equivalent to 212, 1, 3.2, 132 and 1025 nM of 17beta-estradiol, respectively. Controls were performed on soybean and kudzu extracts using specific ELISAs for isoflavones and these confirmed the validity of the cell culture assay. The method described offers an advantage over current methods in that it can detect increased oestrogenic activity that may occur as a result of metabolic activation of pre- or pro-oestrogens liver cells.
体外培养虹鳟鱼肝细胞被证明是一种简单有效的方法，可用于筛选具有雌激素活性的植物和食品。使用该测定法测定了0.1克植物甾醇、大豆异黄酮、红三叶草、葛根和大豆提取物的相对雌激素活性，发现它们分别相当于212、1、3.2、132和1025纳摩尔的17β-雌二醇。使用针对异黄酮的特异性酶联免疫吸附测定法对大豆和葛根提取物进行了对照实验，这些实验证实了细胞培养测定法的有效性。所描述的方法相对于现有方法具有优势，因为它可以检测到由于前雌激素或雌激素原在肝细胞中的代谢激活而可能产生的雌激素活性增加。
