Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.
Br J Nutr. 2009 Nov;102(10):1523-30. doi: 10.1017/S000711450999047X. Epub 2009 Jul 9.
Absorption, distribution and elimination of 14C-labelled isoflavone-containing extracts from kudzu (Pueraria lobata) root culture and red clover (Trifolium pratense) cell culture were investigated in an in vivo rat model. The predominant isoflavones in the kudzu extract were the glycosides puerarin, daidzin and malonyl daidzin, while in the red clover extract, the major isoflavones were formononetin and its derivatives, genistein and biochanin A, with radioactivities of 3.770 and 7.256 MBq/g, respectively. Male Sprague-Dawley rats, implanted with a jugular catheter and a subcutaneous ultrafiltrate probe, were orally administered with 14C-labelled isoflavone extracts from either kudzu or clover cell cultures. Serum, interstitial fluid (ISF), urine and faeces were collected using a Culex Automated Blood Collection System for 24 h. Analysis of bone tissues revealed that radiolabel accumulated in the femur, tibia and vertebrae at 0.04, 0.03 and 0.01 % of the administered dose, respectively, in both kudzu and red clover treatments. The liver accumulated the greatest concentration of radiolabel among the tissues tested, at 1.99 and 1.54 % of the administered kudzu and red clover extracts, respectively. Serum and ISF analysis showed that both extracts were rapidly absorbed, distributed in various tissues, and largely eliminated in the urine and faeces. Urine and faeces contained 8.53 and 9.06 % of the kudzu dose, respectively, and 3.60 and 5.64 % of the red clover dose, respectively. Serum pharmacokinetics suggest that extracts from kudzu may undergo enterohepatic circulation.
在体内大鼠模型中研究了来源于葛根（Pueraria lobata）根培养物和红三叶草（Trifolium pratense）细胞培养物的 14C 标记异黄酮类提取物的吸收、分布和消除。葛根提取物中的主要异黄酮为糖苷葛根素、大豆苷和丙二酰基大豆苷，而红三叶草提取物中的主要异黄酮为芒柄花素及其衍生物染料木黄酮和金雀异黄素，放射性活度分别为 3.770 和 7.256MBq/g。雄性 Sprague-Dawley 大鼠植入颈静脉导管和皮下超滤探头后，经口给予来源于葛根或红三叶草细胞培养物的 14C 标记异黄酮提取物。使用 Culex 自动血液采集系统收集血清、间质液（ISF）、尿液和粪便 24 小时。骨组织分析表明，在葛根和红三叶草处理中，放射性标记物分别在股骨、胫骨和椎骨中累积到给药剂量的 0.04%、0.03%和 0.01%。在测试的组织中，肝脏积累了最大浓度的放射性标记物，分别为给予的葛根和红三叶草提取物的 1.99%和 1.54%。血清和 ISF 分析表明，两种提取物均被快速吸收，分布于各种组织中，并主要从尿液和粪便中消除。尿液和粪便中分别含有葛根剂量的 8.53%和 9.06%，红三叶草剂量的 3.60%和 5.64%。血清药代动力学表明，葛根提取物可能经历肠肝循环。
