Hashempour Zahra, Noraddini Mehdi, Nejati Majid, Jafari Ameneh, Rafiyan Mahdi, Ostadian Amirreza, Alani Behrang, Mirzaei Hamed
Physiology Research Centre, Kashan University of Medical Sciences, Kashan, Iran.
Anatomical Sciences Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Discov Oncol. 2025 Jul 17;16(1):1361. doi: 10.1007/s12672-025-03081-2.
Ovarian cancer (OC) is the leading cause of tumor-related deaths among gynecologic cancers. Patients diagnosed with advanced stages of OC urgently require effective therapeutic interventions, but current therapies often fail in patients with recurrent platinum-resistant tumors. Previous research has shown the cytotoxic effects of Chelidonium majus (C. majus) extract on tumors. In this study we have investigated the effects of combined and independent effects of oxaliplatin and C. majus extract on ovarian cancer cell proliferation and apoptosis. Using the OVACR3 cell line as an in vitro model, we applied the MTT assay for cell growth assessment and RT-qPCR to measure caspase-3, β-catenin, and ZEB1 expression. The results demonstrated that both oxaliplatin and C. majus extract significantly inhibited cell growth and promoted apoptosis, with enhanced effects observed when used together. The inhibition of ZEB1 and β-catenin suggest their involvement in the observed anti-cancer effects. Treated groups also showed higher caspase-3 expression compared to controls. Our findings confirm the synergistic potential of combining oxaliplatin and C. majus extract in treating OC, possibly through the ZEB1/β-catenin pathway. Further research is needed to confirm the clinical applicability and underlying mechanisms of this treatment strategy.
卵巢癌（OC）是妇科癌症中肿瘤相关死亡的主要原因。被诊断为晚期OC的患者迫切需要有效的治疗干预措施，但目前的治疗方法在复发性铂耐药肿瘤患者中往往无效。先前的研究表明白屈菜提取物对肿瘤具有细胞毒性作用。在本研究中，我们研究了奥沙利铂和白屈菜提取物联合及单独作用对卵巢癌细胞增殖和凋亡的影响。以OVACR3细胞系作为体外模型，我们应用MTT法评估细胞生长，并通过RT-qPCR检测caspase-3、β-连环蛋白和ZEB1的表达。结果表明，奥沙利铂和白屈菜提取物均能显著抑制细胞生长并促进凋亡，联合使用时效果增强。ZEB1和β-连环蛋白的抑制表明它们参与了观察到的抗癌作用。与对照组相比，治疗组的caspase-3表达也更高。我们的研究结果证实了奥沙利铂和白屈菜提取物联合治疗OC的协同潜力，可能是通过ZEB1/β-连环蛋白途径。需要进一步研究以证实该治疗策略的临床适用性和潜在机制。
