Kumar P, Agrawal S K, Misra A, Gupta K C
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi-110 007, India.
Bioorg Med Chem Lett. 2004 Mar 8;14(5):1097-9. doi: 10.1016/j.bmcl.2003.12.077.
Synthesis of a new heterobifunctional reagent, [N-(2-trifluoroethanesulfonatoethyl)-N-(methyl)-triethoxysilylpropyl-3-amine] (NTMTA) is described for the immobilization of a variety of biomolecules on glass surface. Its triethoxysilyl group reacts with glass surface and trifluoroethanesulfonate ester structure reacts selectively with aminoalkyl/mercaptoalkyl function in biomolecules. The immobilization can be achieved by two ways involving two steps. The first route involves the reaction of NTMTA with glass beads followed by attachment of aminoalkyl- or mercaptoalkylated biomolecules. The second one involves the reaction of biomolecules, viz., oligonucleotides, proteins, etc., with NTMTA via their aminoalkyl or mercaptoalkyl functions to form a biomolecule conjugate, which is then reacted with glass beads (unmodified) to complete immobilization process. This has been demonstrated by successful immobilization of 5'-mercaptoalkyl- or aminoalkylated oligonucleotides and some commonly used enzymes on glass beads using NTMTA reagent.
描述了一种新型异双功能试剂N-(2-三氟乙烷磺酰基乙基)-N-(甲基)-三乙氧基甲硅烷基丙基-3-胺的合成，用于将多种生物分子固定在玻璃表面。其三乙氧基甲硅烷基与玻璃表面反应，三氟乙烷磺酸酯结构与生物分子中的氨基烷基/巯基烷基官能团选择性反应。固定可通过涉及两个步骤的两种方式实现。第一种途径是NTMTA与玻璃珠反应，然后连接氨基烷基化或巯基烷基化的生物分子。第二种途径是生物分子，即寡核苷酸、蛋白质等，通过其氨基烷基或巯基烷基官能团与NTMTA反应形成生物分子缀合物，然后该缀合物与玻璃珠（未修饰）反应以完成固定过程。使用NTMTA试剂成功地将5'-巯基烷基化或氨基烷基化的寡核苷酸和一些常用酶固定在玻璃珠上，证明了这一点。
