Misra Arvind, Dwivedi Pratibha, Shahid Mohammad
Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India.
Nucleic Acids Symp Ser (Oxf). 2007(51):329-30. doi: 10.1093/nass/nrm165.
An efficient heterobifunctional reagent, N-(3-triethoxysilylpropyl)-4-(N'-maleimidylmethyl) cyclohexanamide (TPMC) was developed for the immobilization of thiol-modified oligonucleotide on the unmodified glass surface. The heterobifuntionality of the reagent was utilized for construction of DNA microarray in which the triethoxysilyl functionality has specificity towards the glass surface while the maleimide functionality for thiol-modified oligonucleotides via stable thio-ether linkage. Immobilization of DNA has been achieved by two alternative approaches. The applicability of the reagent was explored by hybridization studies with the fluorescein labeled complementary DNA strand.
一种高效的异双功能试剂，N-(3-三乙氧基硅丙基)-4-(N'-马来酰亚胺甲基)环己酰胺(TPMC)被开发用于将硫醇修饰的寡核苷酸固定在未修饰的玻璃表面。该试剂的异双功能性被用于构建DNA微阵列，其中三乙氧基硅官能团对玻璃表面具有特异性，而马来酰亚胺官能团则通过稳定的硫醚键与硫醇修饰的寡核苷酸结合。DNA的固定通过两种替代方法实现。通过与荧光素标记的互补DNA链进行杂交研究，探索了该试剂的适用性。
