Herold Susanna
Laboratorium für Anorganische Chemie, Eidgenössische Technische Hochschule, ETH Hönggerberg, Zürich, Switzerland.
Free Radic Biol Med. 2004 Mar 1;36(5):565-79. doi: 10.1016/j.freeradbiomed.2003.10.014.
The biological relevance of tyrosine nitration is a subject of much interest, because extensive evidence supports formation of 3-nitrotyrosine in vivo under a variety of different pathological conditions. Several reagents are likely to be responsible for nitration in vivo, among others peroxynitrite and nitrite in the presence of H(2)O(2)/peroxidases. In this work we show that also metmyoglobin and methemoglobin can nitrate free tyrosine in the presence of nitrite and H(2)O(2). The results of these studies are simulated rather well by using a scheme that comprehends all the possible reactions that can take place in the system. Thus, a good understanding of the factors that determine the yields is achieved. Finally, we demonstrate that the system metMb/H(2)O(2)/NO(2)(-) can also lead to the nitration of tryptophan and produces, in particular, 6-, 4-, and 5-nitrotryptophan.
酪氨酸硝化的生物学相关性是一个备受关注的课题，因为大量证据支持在多种不同病理条件下体内会形成3 - 硝基酪氨酸。体内硝化作用可能由多种试剂引起，其中包括过氧亚硝酸盐以及在过氧化氢/过氧化物酶存在下的亚硝酸盐。在这项工作中，我们表明，在亚硝酸盐和过氧化氢存在的情况下，高铁肌红蛋白和高铁血红蛋白也能使游离酪氨酸硝化。通过使用一个包含系统中所有可能发生反应的方案，相当好地模拟了这些研究结果。因此，对决定产率的因素有了很好的理解。最后，我们证明系统高铁肌红蛋白/过氧化氢/亚硝酸根离子也能导致色氨酸硝化，特别是生成6 - 硝基色氨酸、4 - 硝基色氨酸和5 - 硝基色氨酸。
