Peterfi Z, Churchill L, Hajdu I, Obal F, Krueger J M, Parducz A
Department of Physiology, A. Szent-Györgyi Center, University of Szeged, Hungary.
Neuroscience. 2004;124(3):695-707. doi: 10.1016/j.neuroscience.2003.10.047.
Diurnal variations and sleep deprivation-induced changes in the number of Fos-immunoreactive (Fos-IR) neurons in various hypothalamic/preoptic nuclei were studied in the rat. The nuclei implicated in sleep regulation, the ventrolateral preoptic (VLPO), median preoptic (MnPO), and suprachiasmatic (SCN, dorsomedial subdivision) nuclei, displayed maximum c-fos expression in the rest (light) period. Sleep deprivation (S.D.) suppressed Fos-IR in the dorsomedial subdivision of SCN but failed to alter Fos in the VLPO. Fos-IR increased in the VLPO during recovery after S.D. A nocturnal rise in Fos expression was detected in the arcuate (ARC), anterodorsal preoptic (ADP) and anteroventral periventricular (AVPV) nuclei whereas the lateroanterior hypothalamic nucleus (LA) and the ventrolateral subdivision of SCN did not display diurnal variations. S.D. stimulated Fos expression in the ARC, ADP, and LA. Statistically significant, albeit modest, differences were noted in the number of Fos-IR cells between males and cycling female (estrus/diestrus) in the VLPO, MnPO, ARC, LA, and AVPV, and the female ADP did not display diurnal variations. Ovariectomy (OVX) was followed by marked reduction in Fos expression in the VLPO, SCN, and AVPV, and the diurnal rhythm decreased in the VLPO, and vanished in the dorsomedial SCN, and AVP. Estrogen administration to OVX female rats stimulated Fos expression in most nuclei, and the lost diurnal variations reoccurred. In contrast, castration of male rats had little effect on Fos expression (slight rises in diurnal Fos in the ARC and ventrolateral SCN). The results suggest that Fos expression is highly estrogen-dependent in many hypothalamic nuclei including those that have been implicated in sleep regulation.
在大鼠中研究了昼夜变化以及睡眠剥夺引起的各种下丘脑/视前核中Fos免疫反应性（Fos-IR）神经元数量的变化。参与睡眠调节的核团，即腹外侧视前核（VLPO）、视前正中核（MnPO）和视交叉上核（SCN，背内侧亚区），在休息（光照）期显示出最大的c-fos表达。睡眠剥夺（S.D.）抑制了SCN背内侧亚区的Fos-IR，但未改变VLPO中的Fos。S.D.后恢复期间，VLPO中的Fos-IR增加。在弓状核（ARC）、视前背前核（ADP）和室周前腹核（AVPV）中检测到Fos表达的夜间升高，而下丘脑外侧前核（LA）和SCN腹外侧亚区未显示昼夜变化。S.D.刺激了ARC、ADP和LA中的Fos表达。在VLPO、MnPO、ARC、LA和AVPV中，雄性和处于发情期/间情期的雌性之间Fos-IR细胞数量存在统计学上显著但适度的差异，并且雌性ADP未显示昼夜变化。卵巢切除（OVX）后，VLPO、SCN和AVPV中的Fos表达显著降低，VLPO中的昼夜节律降低，背内侧SCN和AVP中的昼夜节律消失。对OVX雌性大鼠给予雌激素刺激了大多数核团中的Fos表达，并重新出现了消失的昼夜变化。相反，雄性大鼠去势对Fos表达影响很小（ARC和SCN腹外侧昼夜Fos略有升高）。结果表明，Fos表达在许多下丘脑核团中高度依赖雌激素，包括那些参与睡眠调节的核团。
