Ishizuka Yasuko, Nemoto Tadashi, Kanazawa Kenji, Nakanishi Hiroshi
Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Central-6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Carbohydr Res. 2004 Mar 15;339(4):777-85. doi: 10.1016/j.carres.2003.12.021.
The 1H NMR spectra of seven branched alpha-cyclodextrins (alpha-CDs) were observed and analyzed in detail. They were compared with spectra of alpha-CD and amylose. Although these branched alpha-CDs consist only of alpha-D-glucose with the same alpha-(1-->4) O-glucosyl binding, aside from one exception, differences in chemical shifts of corresponding signals were significantly large. Especially, differences in the chemical shift in anomeric protons were considerably large. Subtle differences in glucosyl binding directly influences chemical shifts of these protons because anomeric protons are located adjacent to the glucosyl binding sites.
对七种支链α-环糊精（α-CD）的1H NMR光谱进行了详细的观察和分析。将它们与α-CD和直链淀粉的光谱进行了比较。尽管这些支链α-CD仅由具有相同α-(1→4) O-葡萄糖基连接键的α-D-葡萄糖组成，但除了一个例外，相应信号的化学位移差异非常大。特别是，异头质子的化学位移差异相当大。由于异头质子位于葡萄糖基结合位点附近，葡萄糖基结合的细微差异直接影响这些质子的化学位移。
