Hara K, Fujita K, Kuwahara N, Tanimoto T, Hashimoto H, Koizumi K, Kitahata S
Osaka Municipal Technical Research Institute, Japan.
Biosci Biotechnol Biochem. 1994 Apr;58(4):652-9. doi: 10.1271/bbb.58.652.
Transgalactosylated derivatives of cyclodextrins (CDs) and glucosyl and maltosyl CDs (G1- and G2-CDs) were synthesized by alpha-galactosidases from coffee bean and Mortierella vinacea (M. vinacea). The structures of the transfer products were analyzed by FAB-mass, 13C-NMR and methylation. Coffee bean alpha-galactosidase transferred a galactosyl residue not only to side chains of G1-CDs and G2-CDs, but also directly to CD rings. M. vinacea alpha-galactosidase transferred a galactosyl residue only to side chains of G2-CDs.
通过咖啡豆和葡枝根霉（M. vinacea）中的α-半乳糖苷酶合成了环糊精（CDs）、葡萄糖基环糊精和麦芽糖基环糊精（G1-和G2-CDs）的转半乳糖基化衍生物。通过快原子轰击质谱、13C-核磁共振和甲基化分析了转移产物的结构。咖啡豆α-半乳糖苷酶不仅将半乳糖基残基转移到G1-CDs和G2-CDs的侧链上，还直接转移到CD环上。葡枝根霉α-半乳糖苷酶仅将半乳糖基残基转移到G2-CDs的侧链上。
