Knirel Yuriy A, Vinogradov Evgeny, Jimenez Natalia, Merino Susana, Tomás Juan M
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia.
Carbohydr Res. 2004 Mar 15;339(4):787-93. doi: 10.1016/j.carres.2003.12.008.
A rough strain of Aeromonas hydrophila, AH-901, has an R-type lipopolysaccharide with the complete core. The following core structure was established by chemical degradations followed by sugar and methylation analyses along with ESIMS and NMR spectroscopy: [formula: see text] where D-alpha-D-Hep and l-alpha-D-Hep stand for D-glycero- and l-glycero-alpha-D-manno-heptose, respectively; Kdo stands for 3-deoxy-D-manno-oct-2-ulosonic acid; all monosaccharides are in the pyranose form; the degree of substitution with beta-D-Gal is approximately 50%. Lipid A of the lipopolysaccharide has a 1,4(')-bisphosphorylated beta-D-GlcN-(1-->6)-alpha-D-GlcN disaccharide backbone with both phosphate groups substituted with 4-amino-4-deoxyarabinose residues.
嗜水气单胞菌粗糙菌株AH - 901具有带有完整核心的R型脂多糖。通过化学降解，随后进行糖和甲基化分析以及电喷雾离子化质谱（ESIMS）和核磁共振光谱（NMR），确定了以下核心结构：[化学式：见原文]，其中D-α-D-Hep和L-α-D-Hep分别代表D-甘油基-α-D-甘露庚糖和L-甘油基-α-D-甘露庚糖；Kdo代表3-脱氧-D-甘露辛-2-酮糖酸；所有单糖均为吡喃糖形式；β-D-半乳糖的取代度约为50%。脂多糖的脂质A具有1,4(')-双磷酸化的β-D-葡糖胺-(1→6)-α-D-葡糖胺二糖主链，两个磷酸基团均被4-氨基-4-脱氧阿拉伯糖残基取代。
