诱导型一氧化氮合酶在肾细胞癌中的表达：在肿瘤血栓中的表达及缺氧条件下的诱导作用
Inducible nitric oxide, synthase in renal cell carcinoma: expression in tumor thrombi and induction under hypoxic conditions.
作者信息
Hara Noboru, Bilim Vladimir, Kasahara Takashi, Obara Kenji, Saito Kazuhide, Takahashi Kota, Tomita Yoshihiko
机构信息
Division of Molecular Oncology, Department of Signal Transduction Research, Niigata University Graduate School of Medical and Dental Science, Asahimachi 1, Niigata 951, Japan.
出版信息
Anticancer Res. 2003 Nov-Dec;23(6C):4641-9.
BACKGROUND
Nitric oxide (NO) has been suggested to have polar roles in carcinogenesis with both antitumor and tumor promoting activity, and the status of NO synthase (NOS) in renal cell carcinoma (RCC) has not yet been completely elucidated.
MATERIALS AND METHODS
We investigated the expression and induction of inducible NOS (iNOS) in RCC specimens and cell lines, respectively.
RESULTS
Although the expression of iNOS was not observed in primary lesions or in metastatic sites, it was found in 6 cases of 11 tumor thrombi. The cause-specific survival rate of patients with iNOS-positive tumor thrombi was lower than that of patients with iNOS-negative tumor thrombi, showing borderline significance. iNOS-mRNA and protein were expressed in A498 and A704 RCC cells under hypoxic conditions.
CONCLUSION
iNOS is suggested to be a significant molecule for RCC to acquire not only hypoxic adaptation but also the ability to invade into veins and form tumor thrombi.
背景
一氧化氮（NO）在致癌过程中具有抗肿瘤和促肿瘤的双重作用，而肾细胞癌（RCC）中一氧化氮合酶（NOS）的状态尚未完全阐明。
材料与方法
我们分别研究了RCC标本和细胞系中诱导型NOS（iNOS）的表达和诱导情况。
结果
虽然在原发性病变或转移部位未观察到iNOS的表达，但在11例肿瘤血栓中有6例发现了iNOS。iNOS阳性肿瘤血栓患者的病因特异性生存率低于iNOS阴性肿瘤血栓患者，具有临界显著性。在缺氧条件下，A498和A704 RCC细胞中表达了iNOS-mRNA和蛋白。
结论
iNOS被认为是RCC获得低氧适应性以及侵入静脉并形成肿瘤血栓能力的重要分子。
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