Spees Jeffrey L, Chang Sharon A, Mykles Donald L, Snyder Mark J, Chang Ernest S
Bodega Marine Laboratory, University of California, PO Box 247, Bodega Bay, CA 94923, USA.
Cell Stress Chaperones. 2003 Fall;8(3):258-64. doi: 10.1379/1466-1268(2003)008<0258:mcmcap>2.0.co;2.
Lobster claw muscle undergoes atrophy in correlation with increasing ecdysteroid (steroid molting hormone) titers during premolt. In vivo molecular chaperone (constitutive heat shock protein 70 [Hsc70], heat shock protein 70 [Hsp70], and Hsp90) and polyubiquitin messenger ribonucleic acid (mRNA) levels were examined in claw and abdominal muscles from individual premolt or intermolt lobsters. Polyubiquitin gene expression was assayed as a marker for muscle atrophy. Both Hsc70 and Hsp90 mRNA levels were significantly induced in premolt relative to intermolt lobster claw muscle, whereas Hsp70 mRNA levels were not. Hsp90 gene expression was significantly higher in premolt claw muscle when compared with abdominal muscle. Polyubiquitin mRNA levels were elevated in premolt when compared with intermolt claw muscle and significantly elevated relative to premolt abdominal muscle.
龙虾螯肌在蜕皮前会随着蜕皮类固醇（类固醇蜕皮激素）滴度的增加而发生萎缩。在体内，检测了处于蜕皮前或蜕皮间期的单个龙虾的螯肌和腹肌中分子伴侣（组成型热休克蛋白70 [Hsc70]、热休克蛋白70 [Hsp70] 和Hsp90）以及多聚泛素信使核糖核酸（mRNA）水平。多聚泛素基因表达作为肌肉萎缩的标志物进行检测。相对于蜕皮间期的龙虾螯肌，Hsc70和Hsp90的mRNA水平在蜕皮前均显著上调，而Hsp70的mRNA水平则没有。与腹肌相比，Hsp90基因在蜕皮前的螯肌中表达显著更高。与蜕皮间期的螯肌相比，多聚泛素mRNA水平在蜕皮前升高，并且相对于蜕皮前的腹肌显著升高。
