Ouyang You-Nan, Zhang Qiu-Ying, Zhang Ke-Qin, Yu Sheng-Miao, Zhuang Jie-Yun, Jin Qian-Yu, Cheng Shi-Hua
State Key Laboratory for Rice Biology, National Center of Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.
Yi Chuan Xue Bao. 2005 Jul;32(7):712-8.
A recombinant inbred lines (RILs) population derived from a cross between Zhenshan97B and Miyang46 was used for detecting QTLs with additive effects and additive-by-additive epistasis for rice mesocotyl length. A linkage map consisting of 207 DNA markers,distributing on the 12 chromosomes of rice,was employed for QTL mapping by using software QTL Mapper 1.6 of mixed linear model. Rice mesocotyl length under germination conditions with 4 different FeSO4 concentrations (0, 1.79, 7.16, 14.32 mmol/L) was measured 7 days after planting. A total of 6 QTLs with significant additive effects on chromosome 1, 5 and 9, with variance explained of 3.5%-11.4%, eleven QTLs with significant additive x additive epistatic effects on chromosome 1, 2, 3, 4, 5, 8 were detected, with variance explained of 4.5%-8.1%. In addition, one QTL for environmental interaction (Fe2+ -concentrations) was detected.
以珍汕 97B 和密阳 46 杂交衍生的重组自交系（RILs）群体为材料，检测水稻中胚轴长度的加性效应 QTL 以及加性×加性上位性 QTL。利用分布于水稻 12 条染色体上的 207 个 DNA 标记构建连锁图谱，采用混合线性模型软件 QTL Mapper 1.6 进行 QTL 定位。在种植 7 天后，测定了 4 种不同 FeSO4 浓度（0、1.79、7.16、14.32 mmol/L）发芽条件下的水稻中胚轴长度。共检测到 6 个在第 1、5 和 9 染色体上具有显著加性效应的 QTL，贡献率为 3.5%-11.4%；检测到 11 个在第 1、2、3、4、5、8 染色体上具有显著加性×加性上位性效应的 QTL，贡献率为 4.5%-8.1%。此外，还检测到 1 个与环境互作（Fe2+浓度）相关的 QTL。
