Yuan Ai-Ping, Cao Li-Yong, Zhuang Ji-Yun, Li Run-Zhi, Zheng Kang-Le, Zhu Jun, Cheng Shi-Hua
China National Rice Research Institute, Chinese National Center for Rice Improvement, Hangzhou 310006, China.
Yi Chuan Xue Bao. 2003 Oct;30(10):899-906.
Plant height (PH), heading date (HD) and productive panicle number (PN) are important agronomic trait in rice. Appropriate plant height, heading date and panicle number are prerequisites for the desired high and stable yield level in rice breeding programs. A recombinant inbred line (RIL) population consisting of 304 individuals was derived from a cross between indica varieties Zhong156 and Gumei2, from which a linkage map consisting of 168 RFLP, SSLP, RAPD and RGA markers that distribute on all the 12 rice chromosomes was constructed, and covers 1447.9 cM of the rice genome. The parents and 304 F9 lines were grown in the paddy field in China National Rice Research Institute (CNRRI), Hangzhou, China in 2001. The experiments were carried out in two seasons followed a randomized complete block design. QTLMapper 1.01 was applied to detect QTLs and QTL x environment (QE) interaction for HD (heading data), PH (plant height) and PN (panicle number), and conditional mapping for PH and PN was performed as well. A total of 15 QTLs with significant additive effects were detected, among which 4 QTLs had significant QE interaction. Ten QTLs with additive x additive epistatic effects for PH, HD and PN were detected, among which none showed significant epistatisis x environment interaction. These QTLs explained 12.12%, 1.38% and 5.00% of the total phenotypic variance for PH, HD and PN, respectively, and contributions were generally lower due to the strong epistatic effects. In conditional QTL analysis, the numbers of QTLs showing significant additive and epistatic effects were 7 and 6 for PH, and 3 and 3 for PN, respectively. Among the QTLs having significant additive effects for PH, qPH7-2 showed both additive effects and QE interaction, qPH7-1 and qPH10 showed QE interaction only, and the remaining 4 QTLs showed additive effects only. Each of the 3 QTLs having significant additive effects for PN did not display significant QE interaction. No epistatic QE interactions was detected. In addition, conditional QTL analysis indicated that the expression of QTLs for PH and PN may vary depending on the QTLs for HD.
株高（PH）、抽穗期（HD）和有效穗数（PN）是水稻重要的农艺性状。合适的株高、抽穗期和穗数是水稻育种计划中实现高产稳产的前提条件。一个由304个个体组成的重组自交系（RIL）群体源自籼稻品种中156和谷梅2的杂交，构建了一个由168个RFLP、SSLP、RAPD和RGA标记组成的连锁图谱，这些标记分布在水稻的12条染色体上，覆盖水稻基因组1447.9厘摩。2001年，亲本及304个F9株系在中国杭州的中国水稻研究所（CNRRI）的稻田中种植。试验分两季进行，采用随机完全区组设计。应用QTLMapper 1.01检测HD（抽穗数据）、PH（株高）和PN（穗数）的QTL及QTL与环境（QE）互作，并对PH和PN进行条件作图。共检测到15个具有显著加性效应的QTL，其中4个QTL具有显著的QE互作。检测到10个对PH、HD和PN具有加性×加性上位性效应的QTL，其中没有一个显示出显著的上位性×环境互作。这些QTL分别解释了PH、HD和PN总表型变异的12.12%、1.38%和5.00%，由于上位性效应较强，贡献率普遍较低。在条件QTL分析中，对PH显示显著加性和上位性效应的QTL数量分别为7个和6个，对PN为3个和3个。在对PH具有显著加性效应的QTL中，qPH7-2既显示加性效应又显示QE互作，qPH7-1和qPH10仅显示QE互作，其余4个QTL仅显示加性效应。对PN具有显著加性效应的3个QTL均未显示显著的QE互作。未检测到上位性QE互作。此外，条件QTL分析表明，PH和PN的QTL表达可能因HD的QTL而异。
