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[大白菜(Brassica campestris L. ssp. pekinensis)某些形态性状的数量性状基因座定位与分析]

[Mapping and analysis QTL controlling some morphological traits in Chinese cabbage (Brassica campestris L. ssp. pekinensis)].

作者信息

Yu Shuan-Cang, Wang Yong-Jian, Zheng Xiao-Ying

机构信息

Beijing Vegetable Research Center, Beijing 100089, China.

出版信息

Yi Chuan Xue Bao. 2003 Dec;30(12):1153-60.

PMID:14986434
Abstract

An AFLP and RAPD genetic map with 352 markers and a RIL (recombinant inbred lines) population from the cross of two cultivated Chinese cabbage lines were employed in mapping and analysis quantitative trait loci (QTL). The number, location, variation explained and additive effect of QTL underlying nine morphological traits were determined by using composite interval mapping method. Fifty putative QTL, including five for plant growth habit, six for plant height, five for plant diameter, seven for leaf length, four for leaf width, six for leaf length/leaf width ratio, seven for petiole length, four for petiole width and six for bolting character, were mapped on 14 linkage groups. There were unequal gene effects and unequal variation explained on the expression of many morphological traits. These results are fundamentals for molecular assisted selection of morphological traits in Chinese cabbage breeding.

摘要

利用一个包含352个标记的AFLP和RAPD遗传图谱以及两个大白菜栽培品系杂交产生的重组自交系(RIL)群体,对数量性状位点(QTL)进行定位和分析。采用复合区间作图法确定了九个形态性状相关QTL的数量、位置、变异解释率和加性效应。共定位了50个推定的QTL,包括5个与植株生长习性相关的、6个与株高相关的、5个与株幅相关的、7个与叶片长度相关的、4个与叶片宽度相关的、6个与叶长/叶宽比相关的、7个与叶柄长度相关的、4个与叶柄宽度相关的以及6个与抽薹性状相关的QTL,它们分布在14个连锁群上。许多形态性状的表达存在基因效应不均等和变异解释率不均等的情况。这些结果是大白菜育种中形态性状分子辅助选择的基础。

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