Shrot Yoav, Frydman Lucio
Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
J Magn Reson. 2004 Mar;167(1):42-8. doi: 10.1016/j.jmr.2003.10.008.
We have recently demonstrated that the spatial encoding of internal nuclear magnetic resonance (NMR) spin interactions can be exploited to collect multidimensional NMR spectra within a single scan. Such experiments rely on an inhomogeneous spatial excitation of the spins throughout the sample, and lead to indirect-domain peaks via a constructive interference among the spatially resolved spin-packets that are thus created. The shape of the resulting indirect-domain echo peaks approaches a Sinc function when the chemical's distribution is uniform, but will depart from this function otherwise. It is hereby shown that a Fourier analysis of either the diagonal- or the cross-peaks resolved in these single-scan two-dimensional (2D) NMR experiments can in fact provide a weighted spatial distribution of the analyte originating such peak, thus opening up the possibility of completing spatially resolved multidimensional NMR measurements within a fraction of a second. Principles of this new mode of analysis are discussed, and examples where the potential of spatially resolved ultrafast 2D NMR spectroscopy is brought to bear are presented. Potential extensions of this approach to higher dimensions are also briefly addressed.
我们最近证明，可利用内部核磁共振（NMR）自旋相互作用的空间编码在单次扫描中采集多维NMR谱。此类实验依赖于样品中自旋的非均匀空间激发，并通过由此产生的空间分辨自旋包之间的相长干涉产生间接域峰。当化学物质分布均匀时，所得间接域回波峰的形状接近辛格函数，否则将偏离该函数。本文表明，对这些单次扫描二维（2D）NMR实验中分辨出的对角峰或交叉峰进行傅里叶分析，实际上可以提供产生此类峰的分析物的加权空间分布，从而开辟了在几分之一秒内完成空间分辨多维NMR测量的可能性。讨论了这种新分析模式的原理，并给出了空间分辨超快二维NMR光谱学潜力得以发挥的示例。还简要讨论了将该方法扩展到更高维度的可能性。
