Chang C C, Trosko J E, Akera T
Mutat Res. 1978 Jul;51(1):85-98. doi: 10.1016/0027-5107(78)90011-8.
Ouabain-resistant mutations in Chinese hamster cells have been quantitatively characterized. The mutation frequencies were found to be induced curvilinearly with treatments of increasing doses of ultraviolet light (UV). For the range of UV doses tested (5--20 J/m(2)), the observed frequency, Y, as a function of UV dose X, follows a curvilinear function, Y = (-28 + 13.37 X--1.52X(2) + 0.08X(3)) . 10(-6). The frequencies of UV-induced mutations were directly correlated with cell survival, indicating a similar causal relationship between cell killing and mutation induction. Under the same experimental conditions, X-rays induced 6--thioguanine-, but not ouabain-, resistant mutations. UV-induced ouabain-resistant (ouar) mutants exhibit a selection disadvantage. Their phenotypic expressions are modifiable by various agents. Wild type and 16 ouar mutants were compared with respect to their sensitivity to ouabain inhibition of 86Rb uptake by whole cells. All the ouar mutants assayed are less sensitive to the drug than are wild-type cells. In the absence of ouabain, the Na+--K+--ATPase activities can be significantly higher or lower than that of the wild-type cells.
中国仓鼠细胞中哇巴因抗性突变已得到定量表征。发现突变频率随紫外线（UV）剂量增加呈曲线诱导。对于所测试的紫外线剂量范围（5--20 J/m(2)），观察到的频率Y作为紫外线剂量X的函数，遵循曲线函数Y = (-28 + 13.37X - 1.52X(2) + 0.08X(3))×10(-6)。紫外线诱导的突变频率与细胞存活直接相关，表明细胞杀伤与突变诱导之间存在相似的因果关系。在相同实验条件下，X射线诱导6-硫鸟嘌呤抗性突变，但不诱导哇巴因抗性突变。紫外线诱导的哇巴因抗性（ouar）突变体表现出选择劣势。它们的表型表达可被各种试剂修饰。比较了野生型和16个ouar突变体对哇巴因抑制全细胞摄取86Rb的敏感性。所有检测的ouar突变体对该药物的敏感性均低于野生型细胞。在没有哇巴因的情况下，Na+ - K+ - ATP酶活性可能显著高于或低于野生型细胞。
