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SURVIVAL KINETICS OF HELA S-3 CELLS AFTER INCORPORATION OF 3H-THYMIDINE OR 3H-URIDINE.掺入³H-胸腺嘧啶核苷或³H-尿苷后海拉S-3细胞的存活动力学
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Evidence for transfer of enzyme product as the basis of metabolic cooperation between tissue culture fibroblasts of Lesch-Nyhan disease and normal cells.作为莱施-奈恩病组织培养成纤维细胞与正常细胞之间代谢合作基础的酶产物转移的证据。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1573-9. doi: 10.1073/pnas.67.3.1573.
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Biological damage from intranuclear tritium: DNA strand breaks and their repair.细胞核内氚造成的生物损伤：DNA链断裂及其修复
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4
A comparison of the 8-azaguanine and ouabain-resistance systems for the selection of induced mutant Chinese hamster cells.用于筛选诱导突变的中国仓鼠细胞的8-氮杂鸟嘌呤和哇巴因抗性系统的比较。
Mutat Res. 1975 Dec;33(2-3):261-78. doi: 10.1016/0027-5107(75)90202-x.
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DNA crosslinks, single-strand breaks and effects on bacteriophage T4 survival from tritium decay of (2-3H)adenine, (8-3H)adenine and (8-3H)guanine.
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3H-胸腺嘧啶核苷诱导中国仓鼠卵巢细胞DNA中硫鸟嘌呤和哇巴因抗性突变体及单链断裂

Induction of thioguanine- and ouabain-resistant mutants and single-strand breaks in the DNA of Chinese hamster ovary cells by 3H-thymidine.

作者信息

Cleaver J E

出版信息

Genetics. 1977 Sep;87(1):129-38. doi: 10.1093/genetics/87.1.129.

DOI:10.1093/genetics/87.1.129
PMID:914028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1213721/
Abstract

Cultured Chinese hamster cells were labeled with 6-3H-thymidine or 5-methyl-3H-thymidine and allowed to accumulate damage from 3H decays for various periods of time while frozen. The frequencies of cells resistant to 6-thioguanine or ouabain and the amount of DNA damage (i.e., number of single-strand breaks) were determined and compared with the mutation frequencies resulting from X and ultraviolet light irradiation. Whereas 3H decays and X rays made only 6-thioguanine-resistant mutants, ultraviolet light made both 6-thioguanine- and ouabain-resistant mutants. 3H decays originating at the 6 position were two to three times as effective as decays at the 5-methyl position in making drug-resistant mutants, but decays at both sites were equally effective in making single-strand breaks. Mutants and strand breaks produced by beta irradiation of the nucleus probably are the same irrespective of the site of the decay in thymine; these results indicate that the local transmutation effects of 3H decay produce more mutations when they occur at the 6 position than at the 5-methyl position.

摘要

将培养的中国仓鼠细胞用6-³H-胸腺嘧啶核苷或5-甲基-³H-胸腺嘧啶核苷进行标记，并在冷冻状态下让其在不同时间段内累积³H衰变造成的损伤。测定对6-硫鸟嘌呤或哇巴因具有抗性的细胞频率以及DNA损伤量（即单链断裂数），并与X射线和紫外线照射产生的突变频率进行比较。³H衰变和X射线仅产生对6-硫鸟嘌呤具有抗性的突变体，而紫外线则产生对6-硫鸟嘌呤和哇巴因均具有抗性的突变体。在产生抗药突变体方面，源自6位的³H衰变比5-甲基位的衰变有效两到三倍，但两个位点的衰变在产生单链断裂方面同样有效。无论胸腺嘧啶中衰变位点如何，由细胞核的β辐射产生的突变体和链断裂可能是相同的；这些结果表明，³H衰变的局部嬗变效应在6位发生时比在5-甲基位发生时产生更多突变。